Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

Poor
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (immunoprecipitated b-catenin from mES cells)
Loading amount
1e+006 cells
Specification
immunoprecipitated b-catenin from mES cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/50
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5%BSA in TBST

Secondary antibody

Name
Non-Abcam antibody was used: licor anti rabbit igG 680
Host species: Goat
Clonality: Monoclonal
Conjugation: Other
Dilution
1/10000

Detection

Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: many kDa
Negative control
b-catenin null mES cells

Additional data

Additional Notes
this experiment was done with the licor system, here ab27798 was detected (or not detected as the reveiw states) in the 800 channel, in the 680 chanel I detected b-catenin with ab19450, this suggests that the AB is not working in these conditions.
BSA used for blocking as Milk can inhibit/ reduce the efficiency of antibodies detecting phospho epitopes
Abcam response
Thank you for testing ab24925 via IP and for your honest feedback. As this antibody has not been previously tested in IP it may be that it is not effective in this application.

Dr. Penny Hayward

Verified customer

Submitted Jan 17 2012

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