Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (pancreas)
Loading amount
30 µg
Specification
pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/500
Incubation time
8 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: peroxidase conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 90 kDa

Additional data

Additional Notes
no non-specific bands obtained

Dr. Alessandra Strom

Verified customer

Submitted May 10 2006

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