Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela S3 cells)
Loading amount
30 µg
Specification
Hela S3 cells
Treatment
+/- [30 mM] Pervanadate for 30 min
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Supersignal West Dura
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: none (species at 120 kDa
Positive control
SW480 lysate treated with Pervanadate (Supplied by Abcam)

Additional data

Additional Notes
This antibody recognizes a band of the predicted mobility in both the positive control lysate supplied by Abcam (lane 5), and in 3T3 cells stimulated with Pervanadate for 30 mins. A weak signal was observed in Hela S3 cells, which titrates with the reduced expression of B-catenin in this celll line.
This antibody is clean, but may require a long exposure.

Lane Order: (1) HeLa S3: Control (2) HeLa S3: Pervanadate 30 min (3) BalbC/3T3: Control (4) BalbC/3T3: Pervanadate 30 min (5) Pervanadate treated SW480 lysate

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Submitted Nov 01 2007

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