Specific: 90 kDa Non-specific: none (species at 120 kDa
SW480 lysate treated with Pervanadate (Supplied by Abcam)
This antibody recognizes a band of the predicted mobility in both the positive control lysate supplied by Abcam (lane 5), and in 3T3 cells stimulated with Pervanadate for 30 mins. A weak signal was observed in Hela S3 cells, which titrates with the reduced expression of B-catenin in this celll line. This antibody is clean, but may require a long exposure.
Lane Order: (1) HeLa S3: Control (2) HeLa S3: Pervanadate 30 min (3) BalbC/3T3: Control (4) BalbC/3T3: Pervanadate 30 min (5) Pervanadate treated SW480 lysate
Abcam user community
Submitted Nov 01 2007
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