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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-beta Catenin (phospho Y333) antibody (ab194797)

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Western blot - Anti-beta Catenin (phospho Y333) antibody (ab194797)

    Key features and details

    • Rabbit polyclonal to beta Catenin (phospho Y333)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant Human beta Catenin protein (Tagged) (ab63175)

    View more associated products

    Overview

    • Product name

      Anti-beta Catenin (phospho Y333) antibody
      See all beta Catenin primary antibodies
    • Description

      Rabbit polyclonal to beta Catenin (phospho Y333)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human beta Catenin (phospho Y333). The exact sequence is proprietary.
      Database link: P35222

    • Positive control

      • 293 cell extract treated with Forskolin
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cadherins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Catenins
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Cadherins
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human beta Catenin protein (Tagged) (ab63175)

    Applications

    Our Abpromise guarantee covers the use of ab194797 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 85 kDa.

    Target

    • Function

      Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
      Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
    • Tissue specificity

      Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
    • Involvement in disease

      Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
      Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
      Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
      Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
      Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
      Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
    • Sequence similarities

      Belongs to the beta-catenin family.
      Contains 12 ARM repeats.
    • Post-translational
      modifications

      Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
      EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
      Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
    • Target information above from: UniProt accession P35222 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1499 Human
      • Omim: 116806 Human
      • SwissProt: P35222 Human
      • Unigene: 476018 Human
      • Alternative names

        • b-catenin antibody
        • Beta catenin antibody
        • Beta-catenin antibody
        • Cadherin associated protein antibody
        • Catenin (cadherin associated protein), beta 1, 88kDa antibody
        • Catenin beta 1 antibody
        • Catenin beta-1 antibody
        • CATNB antibody
        • CHBCAT antibody
        • CTNB1_HUMAN antibody
        • CTNNB antibody
        • CTNNB1 antibody
        • DKFZp686D02253 antibody
        • FLJ25606 antibody
        • FLJ37923 antibody
        • OTTHUMP00000162082 antibody
        • OTTHUMP00000165222 antibody
        • OTTHUMP00000165223 antibody
        • OTTHUMP00000209288 antibody
        • OTTHUMP00000209289 antibody
        see all

      Images

      • Western blot - Anti-beta Catenin (phospho Y333) antibody (ab194797)
        Western blot - Anti-beta Catenin (phospho Y333) antibody (ab194797)
        All lanes : Anti-beta Catenin (phospho Y333) antibody (ab194797) at 1/500 dilution

        Lane 1 : 293 cell extract untreated
        Lane 2 : 293 cell extract treated with Forskolin

        Predicted band size: 85 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab194797? Please let us know so that we can cite the reference in this datasheet.

      ab194797 has not yet been referenced specifically in any publications.

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