• Product name

    beta Catenin (pS45 + Total) ELISA Kit
  • Detection method

  • Precision

    Sample n Mean SD CV%
    A459 (pS45) 6 3.1%
    A459 (Total) 6 1.7%
    Sample n Mean SD CV%
    A459 (pS45) 3 3.4%
    A459 (Total) 3 3.2%
  • Sample type

    Cell culture extracts, Cell Lysate
  • Assay type

  • Sensitivity

    2 µg/ml
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    Abcam’s β-Catenin (pS45) and β-Catenin (Total) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit (ab205705) is designed for the semi-quantitative measurement of β-Catenin (pS45) and Total β-Catenin protein in Human and mouse cells. β-Catenin is part of a complex of proteins that constitute adherens junctions, and play an important role mediating cellular adhesion. β-Catenin is also a key downstream effector in the Wnt signaling pathway.


    An alternative fluorescent substrate, ADHP, can be used with this assay. A microplate reader capable of measuring fluorescence is required if using this product.

  • Notes

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)



Our Abpromise guarantee covers the use of ab205705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.


  • Cell line analysis for Total β-Catenin from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

  • Activation of β-Catenin (pS45) phosphorylation in A549 cells in response to calyculin treatment. A549 cells were cultured in 96-well tissue culture plates and treated (30 minutes) with a dose-range of calyculin before cell lysis. Data from quadruplicate measurements of β-Catenin (pS45) are plotted and compared against total β-Catenin protein levels.

  • Example of a typical β-Catenin (pS45) and β-Catenin (Total) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed



ab205705 has not yet been referenced specifically in any publications.

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