Overview

  • Product name

  • Description

    Rabbit polyclonal to beta COP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla
  • Immunogen

    Synthetic peptide within Human beta COP aa 853-903. The exact sequence is proprietary.
    Sequence:

    VTVNTNMV DLNDYLQHIL KSTNMKCLTP EKALSGYCGF MAANLYARSI FGE


    Database link: NP_001137533.1

  • Positive control

    • HeLa, 293T, Jurkat, TCMK and NIH-3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab205020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
  • Sequence similarities

    Contains 6 HEAT repeats.
  • Post-translational
    modifications

    Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. Cell membrane. Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta-coat protein antibody
    • Beta-COP antibody
    • betacop antibody
    • Coatomer beta subunit antibody
    • Coatomer protein complex subunit beta 1 antibody
    • Coatomer protein complex subunit beta antibody
    • Coatomer subunit beta antibody
    • COPB antibody
    • COPB_HUMAN antibody
    • Copb1 antibody
    • DKFZp761K102 antibody
    • FLJ10341 antibody
    see all

Images

  • Detection of beta COP in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205020 at 6 µg/mg lysate for IP (Lane 2). For WB detection an ab205017 was used at 0.4 µg/mL. Lane 1 represents positive control and lane 3 is control IgG IP.

  • All lanes : Anti-beta COP antibody (ab205020) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK whole cell lysate
    Lane 5 : NIH-3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 107 kDa


    Exposure time: 3 seconds

References

ab205020 has not yet been referenced specifically in any publications.

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