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RecombinantRabMAb

Recombinant Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)

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Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)
  • Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9585] to beta Crystallin A3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-beta Crystallin A3 antibody [EPR9585]
    See all beta Crystallin A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9585] to beta Crystallin A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human beta Crystallin A3 (UniProt: P05813).

  • Positive control

    • Mouse and Rat eyeball lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9585
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Visual system

Associated products

  • Alternative Versions

    • Anti-beta Crystallin A3 antibody [EPR9585] - BSA and Azide free (ab249032)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab151713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Crystallins are the dominant structural components of the vertebrate eye lens.
    • Involvement in disease

      Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens.
    • Sequence similarities

      Belongs to the beta/gamma-crystallin family.
      Contains 4 beta/gamma crystallin 'Greek key' domains.
    • Domain

      Has a two-domain beta-structure, folded into four very similar Greek key motifs.
    • Post-translational
      modifications

      Specific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
      S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
      Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated.
    • Target information above from: UniProt accession P05813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1411 Human
      • Entrez Gene: 12957 Mouse
      • Entrez Gene: 25583 Rat
      • Omim: 123610 Human
      • SwissProt: P05813 Human
      • SwissProt: P02525 Mouse
      • SwissProt: P14881 Rat
      • Unigene: 46275 Human
      • Unigene: 22830 Mouse
      • Unigene: 20326 Rat
      see all
    • Alternative names

      • Beta crystallin A3 isoform A1 Delta4 form antibody
      • Beta crystallin A3 antibody
      • Beta crystallin A3 isoform A1 Delta7 form antibody
      • Beta crystallin A3 isoform A1 Delta8 form antibody
      • Beta-crystallin A3 antibody
      • CRBA1_HUMAN antibody
      • CRYB1 antibody
      • CRYBA1 antibody
      • Crystallin beta A1 antibody
      • Crystallin beta A3 antibody
      • Delta8 form antibody
      • Eye lens structural protein antibody
      • isoform A1 antibody
      see all

    Images

    • Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)
      Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)
      All lanes : Anti-beta Crystallin A3 antibody [EPR9585] (ab151713) at 1/1000 dilution

      Lane 1 : Mouse eyeball lysate
      Lane 2 : Rat eyeball lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)
      Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151713? Please let us know so that we can cite the reference in this datasheet.

    ab151713 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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