Recombinant
Recombinant Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-beta Crystallin A3 antibody [EPR9585]
See all beta Crystallin A3 primary antibodies -
Description
Rabbit monoclonal [EPR9585] to beta Crystallin A3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide, corresponding to residues in Human beta Crystallin A3 (UniProt: P05813).
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Positive control
- Mouse and Rat eyeball lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9585 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
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Function
Crystallins are the dominant structural components of the vertebrate eye lens. -
Involvement in disease
Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens. -
Sequence similarities
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains. -
Domain
Has a two-domain beta-structure, folded into four very similar Greek key motifs. -
Post-translational
modificationsSpecific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated. - Information by UniProt
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Database links
- Entrez Gene: 1411 Human
- Entrez Gene: 12957 Mouse
- Entrez Gene: 25583 Rat
- Omim: 123610 Human
- SwissProt: P05813 Human
- SwissProt: P02525 Mouse
- SwissProt: P14881 Rat
- Unigene: 46275 Human
see all -
Alternative names
- Beta crystallin A3 isoform A1 Delta4 form antibody
- Beta crystallin A3 antibody
- Beta crystallin A3 isoform A1 Delta7 form antibody
see all
Images
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![Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)](https://www.abcam.com/ps/datasheet/images/151/ab151713/beta-Crystallin-A3-Primary-antibodies-ab151713-1.jpg)
Western blot - Anti-beta Crystallin A3 antibody [EPR9585] (ab151713)All lanes : Anti-beta Crystallin A3 antibody [EPR9585] (ab151713) at 1/1000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Protocols
Datasheets and documents
References
ab151713 has not yet been referenced specifically in any publications.
