Recombinant Anti-beta Crystallin A3 antibody [EPR9585] - BSA and Azide free (ab249032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9585] to beta Crystallin A3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-beta Crystallin A3 antibody [EPR9585] - BSA and Azide free
See all beta Crystallin A3 primary antibodies -
Description
Rabbit monoclonal [EPR9585] to beta Crystallin A3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249032 is the carrier-free version of ab151713.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9585 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
Target
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Function
Crystallins are the dominant structural components of the vertebrate eye lens. -
Involvement in disease
Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens. -
Sequence similarities
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains. -
Domain
Has a two-domain beta-structure, folded into four very similar Greek key motifs. -
Post-translational
modificationsSpecific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated. - Information by UniProt
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Database links
- Entrez Gene: 1411 Human
- Entrez Gene: 12957 Mouse
- Entrez Gene: 25583 Rat
- Omim: 123610 Human
- SwissProt: P05813 Human
- SwissProt: P02525 Mouse
- SwissProt: P14881 Rat
- Unigene: 46275 Human
see all -
Alternative names
- Beta crystallin A3 isoform A1 Delta4 form antibody
- Beta crystallin A3 antibody
- Beta crystallin A3 isoform A1 Delta7 form antibody
see all
Images
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All lanes : Anti-beta Crystallin A3 antibody [EPR9585] (ab151713) at 1/1000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDaThis data was developed using ab151713, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249032 has not yet been referenced specifically in any publications.