Recombinant
RabMAb

Recombinant Anti-beta Crystallin A3 antibody [EPR9586] (ab151722)

Overview

  • Product name

    Anti-beta Crystallin A3 antibody [EPR9586]
    See all beta Crystallin A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9586] to beta Crystallin A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human beta Crystallin A3 (UniProt: P05813).

  • Positive control

    • Mouse and Rat eyeball lysates; Y79 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9586
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Crystallins are the dominant structural components of the vertebrate eye lens.
    • Involvement in disease

      Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens.
    • Sequence similarities

      Belongs to the beta/gamma-crystallin family.
      Contains 4 beta/gamma crystallin 'Greek key' domains.
    • Domain

      Has a two-domain beta-structure, folded into four very similar Greek key motifs.
    • Post-translational
      modifications

      Specific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
      S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
      Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beta crystallin A3 isoform A1 Delta4 form antibody
      • Beta crystallin A3 antibody
      • Beta crystallin A3 isoform A1 Delta7 form antibody
      • Beta crystallin A3 isoform A1 Delta8 form antibody
      • Beta-crystallin A3 antibody
      • CRBA1_HUMAN antibody
      • CRYB1 antibody
      • CRYBA1 antibody
      • Crystallin beta A1 antibody
      • Crystallin beta A3 antibody
      • Delta8 form antibody
      • Eye lens structural protein antibody
      • isoform A1 antibody
      see all

    Images

    • All lanes : Anti-beta Crystallin A3 antibody [EPR9586] (ab151722) at 1/10000 dilution

      Lane 1 : Mouse eyeball lysate
      Lane 2 : Rat eyeball lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunofluorescent analysis of Y79 cells labeling beta Crystallin A3 with ab151722 at 1/50 dilution.

    References

    This product has been referenced in:

    • Ghosh S  et al. A Role for ßA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 59:AMD104-AMD113 (2018). Read more (PubMed: 30098172) »
    • Choudhary P  et al. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage. Stem Cells Transl Med 6:490-501 (2017). Read more (PubMed: 28191760) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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