Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9586] to beta Crystallin A3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-beta Crystallin A3 antibody [EPR9586]
See all beta Crystallin A3 primary antibodies
DescriptionRabbit monoclonal [EPR9586] to beta Crystallin A3
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human beta Crystallin A3 (UniProt: P05813).
- Mouse and Rat eyeball lysates; Y79 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab151722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
Involvement in diseaseDefects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens.
Sequence similaritiesBelongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains.
DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
modificationsSpecific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated.
- Information by UniProt
- Beta crystallin A3 isoform A1 Delta4 form antibody
- Beta crystallin A3 antibody
- Beta crystallin A3 isoform A1 Delta7 form antibody
All lanes : Anti-beta Crystallin A3 antibody [EPR9586] (ab151722) at 1/10000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Immunofluorescent analysis of Y79 cells labeling beta Crystallin A3 with ab151722 at 1/50 dilution.
ab151722 has been referenced in 3 publications.
- Lennikov A et al. Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation. Front Immunol 10:1903 (2019). PubMed: 31474986
- Ghosh S et al. A Role for ßA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 59:AMD104-AMD113 (2018). PubMed: 30098172
- Choudhary P et al. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage. Stem Cells Transl Med 6:490-501 (2017). PubMed: 28191760