Key features and details
- Rabbit polyclonal to beta Defensin 1 (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-beta Defensin 1 antibody (Biotin)
See all beta Defensin 1 primary antibodies
DescriptionRabbit polyclonal to beta Defensin 1 (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant Human beta Defensin 1
FormLyophilized:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab84245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Use 100 μl/well antibody solution. Detects of at least 0.2 – 0.4 ng/well of recombinant human beta Defensin 1.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.
The detection limit for recombinant human beta Defensin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionHas bactericidal activity.
Sequence similaritiesBelongs to the beta-defensin family.
- Information by UniProt
- BD 1 antibody
- BD-1 antibody
- BD1 antibody
To detect human beta Defensin 1 by Western Blot analysis ab84245 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human beta Defensin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant beta Defensin 1 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant beta Defensin 1 protein.
To detect human beta Defensin 1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84245 is required. This biotinylated polyclonal antibody, in conjunction with an anti-beta Defensin 1 (ab109571) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human beta Defensin 1.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84245 has been referenced in 1 publication.
- Trend S et al. Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis. PLoS One 10:e0117038 (2015). ELISA . PubMed: 25643281