• Product name
    Anti-beta Defensin 3 antibody
    See all beta Defensin 3 primary antibodies
  • Description
    Rabbit polyclonal to beta Defensin 3
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant full length human beta defensin 3.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab19270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23627872


  • Function
    Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
  • Tissue specificity
    Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
  • Sequence similarities
    Belongs to the beta-defensin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 3 antibody
    • BD-3 antibody
    • BD3 antibody
    • beta 103 antibody
    • Beta defensin 103A antibody
    • Beta defensin 103A precursor antibody
    • Beta defensin 3 antibody
    • Beta-defensin 103 antibody
    • Beta-defensin 3 antibody
    • D103A_HUMAN antibody
    • DEF B3 antibody
    • DEFB 103 antibody
    • DEFB 103A antibody
    • DEFB 3 antibody
    • DEFB-3 antibody
    • DEFB103 antibody
    • DEFB103A antibody
    • DEFB103B antibody
    • DEFB3 antibody
    • Defensin antibody
    • Defensin beta 103A antibody
    • Defensin beta 3 antibody
    • Defensin like protein antibody
    • Defensin-like protein antibody
    • HBD 3 antibody
    • hBD-3 antibody
    • HBD3 antibody
    • HBP 3 antibody
    • HBP3 antibody
    • mBD3 antibody
    see all


  • ab19270 staining beta Defensin 3 in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An  Alexa Fluor®488-conjugated  was used as the secondary antibody and DAPI used to stain the nuclei.


This product has been referenced in:
  • Zhu M  et al. Expression and antimicrobial character of cells transfected with human ß-defensin-3 against periodontitis-associated microbiota in vitro. Mol Med Rep 16:2455-2460 (2017). Read more (PubMed: 28677765) »
  • Pierson T  et al. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis 11:10 (2013). ICC/IF ; Human . Read more (PubMed: 23627872) »

See all 3 Publications for this product

Customer reviews and Q&As

Sure, I am happy to send along the replacements.

If you could please remind me of the purchase order number or Abcam order reference number associated with the original purchase of these antibodies, I'll arrange replacements immediately.
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Immunohistochemistry (Frozen sections)
Equus Tissue sections (respiratory tract)
Yes - 0.1% Triton X 100
respiratory tract
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Jolien Van Cleemput

Verified customer

Submitted Dec 07 2015

Does your customer want to use the same catalog numbers or would she be interested in choosing one or more different antibodies (of the same price) to the beta defensin proteins?

Thanks for passing along the peptide and protein concentration information.

These concentrations seem reasonable.

Is your customer interested in replacements for the antibodies?

Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

Here are my thoughts:

1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

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Thank you for your enquiry. The immunogen was recombinant human beta-defensin-3. This same material was used in Western blots using an SDS-PAGE mini gel. I have updated the datasheet to reflect that the immunogen is recombinant. Please let us k...

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