Anti-beta Defensin 3 antibody [L3-18b-E1] (ab14422)


  • Product name
    Anti-beta Defensin 3 antibody [L3-18b-E1]
    See all beta Defensin 3 primary antibodies
  • Description
    Mouse monoclonal [L3-18b-E1] to beta Defensin 3
  • Host species
  • Tested applications
    Suitable for: ELISA, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 6-22 of Human beta 3 Defensin .



Our Abpromise guarantee covers the use of ab14422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21056450


  • Function
    Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
  • Tissue specificity
    Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
  • Sequence similarities
    Belongs to the beta-defensin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 3 antibody
    • BD-3 antibody
    • BD3 antibody
    • beta 103 antibody
    • Beta defensin 103A antibody
    • Beta defensin 103A precursor antibody
    • Beta defensin 3 antibody
    • Beta-defensin 103 antibody
    • Beta-defensin 3 antibody
    • D103A_HUMAN antibody
    • DEF B3 antibody
    • DEFB 103 antibody
    • DEFB 103A antibody
    • DEFB 3 antibody
    • DEFB-3 antibody
    • DEFB103 antibody
    • DEFB103A antibody
    • DEFB103B antibody
    • DEFB3 antibody
    • Defensin antibody
    • Defensin beta 103A antibody
    • Defensin beta 3 antibody
    • Defensin like protein antibody
    • Defensin-like protein antibody
    • HBD 3 antibody
    • hBD-3 antibody
    • HBD3 antibody
    • HBP 3 antibody
    • HBP3 antibody
    • mBD3 antibody
    see all


This product has been referenced in:
  • Yost S  et al. Potassium is a key signal in host-microbiome dysbiosis in periodontitis. PLoS Pathog 13:e1006457 (2017). IHC . Read more (PubMed: 28632755) »
  • Tugizov SM  et al. HIV is inactivated after transepithelial migration via adult oral epithelial cells but not fetal epithelial cells. Virology 409:211-22 (2011). IHC-Fr ; Human . Read more (PubMed: 21056450) »

See all 2 Publications for this product

Customer reviews and Q&As

Following up my previous message from Wednesday, I have received the following protocol from the laboratory that valdiated this antibody for ELISA: We tested the antibody in ELISA with the following conditions: Coating of plate with 100 µl of (2 ...

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Thank you for the datasheets of the secondary antibodies. I am surprised that Thermo recommended 31462. The datasheet for this secondary includes this note: The product has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-rea...

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Thank you for the clarification. I have just  a few more questions. 1. In the first experiment, did the anti-mouse secondary react with ab14422? 2. What is the source and catalogue number for the anti-rabbit secondary that reacted with ab14422? 3. For ...

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Thank you for the clarification. We are confident that ab14422 is a mouse monoclonal antibody. Have you used the secondary with other mouse antibodies, isotype IgG1? That is the isotype (IgG1) of this clone (L3-18b-E1). I recommend contacting the compa...

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Thank you for contacting us. The BD-3 antibody is a mouse monoclonal antibody. Is the anti-rabbit secondary also an Abcam antibody? If it has not been pre-adsorbed to remove reactivity with mouse IgG, it is possible it cross-reacts with mouse I...

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Thank you for getting back to me with those details, it was very interesting. Unfortunately given that the source of the antisera have not been able to provide me with further details as to the synthesis of the immunising peptides it is difficult f...

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Thank you for your enquiry. Further to correspondence with the source of this antiserum I have been informed that the antiserum was raised against tricycle human ß-Defensin 2 antigen. Unfortunately they could not provide details of whether the anti...

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Thank you for contacting me. Unfortunately I have not been able to obtain a satisfactory answer from the source of this antiserum as yet. I will be in touch as soon as I know more details about the synthesis of the immunising peptides. I apprec...

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