Key features and details
- Rabbit monoclonal [EP2200Y] to beta Dystroglycan
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-beta Dystroglycan antibody [EP2200Y]
See all beta Dystroglycan primary antibodies
DescriptionRabbit monoclonal [EP2200Y] to beta Dystroglycan
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human beta Dystroglycan aa 850-950 (C terminal). The exact sequence is proprietary.
- WB: Human brain tissue lysate; mouse brain, heart, and skeletal muscle tissue lysates; HEK293T, Neuro2a and NIH/3T3 whole cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab62373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 43 kDa.
Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
RelevanceDystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
Cellular localizationType I membrane protein.
- 156DAG antibody
- A3a antibody
- AGRNR antibody
All lanes : Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAG1 knockout HEK293T cell lysate
Lane 3 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 43 kDa why is the actual band size different from the predicted?
Lanes 1-3: Merged signal (red and green). Green - ab62373 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab62373 Anti-beta Dystroglycan antibody [EP2200Y] was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab62373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 0.526 µg/ml (Purified)
Lane 1 : Mouse brain lysate with 5% NFDM /TBST
Lane 2 : Mouse heart lysate with 5% NFDM /TBST
Lane 3 : Mouse skeletal muscle with 5% NFDM /TBST
Lane 4 : Neuro2a (Mouse neuroblastoma neuroblast) with 5% NFDM /TBST
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with 5% NFDM /TBST
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/2000 dilution + human brain lysate at 10 µg
Goat anti-rabbit, HRP labeled, at 1/2000 dilution
Molecular weight 43kDa.
ab62373 has not yet been referenced specifically in any publications.