Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-beta Dystroglycan (phospho Y892) antibody [M117]
See all beta Dystroglycan primary antibodies -
DescriptionMouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
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Host speciesMouse
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SpecificityAb29037 recognises beta Dystroglycan (phospho Y892).
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.
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Positive control
- HepG2 cells or HeLa cells treated with pervanadate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberM117
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab29037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
ELISA: 1/2000.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceDystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
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Cellular localizationType I membrane protein.
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Database links
- Entrez Gene: 1605 Human
- Entrez Gene: 13138 Mouse
- Entrez Gene: 114489 Rat
- Omim: 128239 Human
- SwissProt: Q14118 Human
- SwissProt: Q62165 Mouse
- Unigene: 76111 Human
- Unigene: 36260 Rat
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Alternative names
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
Images
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![Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](https://www.abcam.com/ps/products/29/ab29037/Images/ab29037_2.jpg)
Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
Lane 1 : Lysates from HepG2 cells (untreated)
Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa. -
![Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)](https://www.abcam.com/ps/products/29/ab29037/Images/ab29037_3.jpg)
Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).
Protocols
References
ab29037 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"