Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

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Overview

  • Product name

    Anti-beta Dystroglycan (phospho Y892) antibody [M117]
    See all beta Dystroglycan primary antibodies

  • Description

    Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)

  • Host species

    Mouse

  • Specificity

    Ab29037 recognises beta Dystroglycan (phospho Y892).

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 892 of human dystroglycan.

  • Positive control

    • HepG2 cells or HeLa cells treated with pervanadate.

Properties

Applications

Our Abpromise guarantee covers the use of ab29037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.

    • Cellular localization

      Type I membrane protein.

    • Database links

      • Alternative names

        • 156DAG antibody
        • A3a antibody
        • AGRNR antibody
        • Beta DG antibody
        • Beta dystroglycan antibody
        • DAG 1 antibody
        • DAG1 antibody
        • Dystroglycan antibody
        • Dystrophin associated glycoprotein 1 antibody
        see all

      Images

      • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

        Lane 1 : Lysates from HepG2 cells (untreated)
        Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min

        Lysates/proteins at 20 µg per lane.

        Predicted band size: 97 kDa
        Observed band size: 43 kDa
        why is the actual band size different from the predicted?



        Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa.
      • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
        Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).

      References

      ab29037 has not yet been referenced specifically in any publications.

      Publishing research using ab29037? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Question

      I am currently investigating the antibodies against dystroglycan both alpha and beta and have seen that you have a large selection of different clones available. The antibodies with product codes mentioned above state they have not been referenced in papers yet... is this information up to date or are there still no references or evidence that these antibodies work. If this is the case it would be helpful if you could forward any information/evidence that you that these work. I would also be happy to test them if you were able to send them free of charge.

      Thank you for any assistance you can provide,

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      Answer

      Thank you for your inquiry.

      I can confirm that we do not know of any publication using the above mentioned antibodies. I would like to reassure you that we do guarantee our product for all species and applications stated on the respective datasheet.

      If you would like to use one of our products in a species or application that it has not been previously been tested in, you might be eligible for our testing discount program.

      https://www.abcam.com/collaborationdiscount

      For more information please see below:

      At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.

      The Abcam Abpromise® guarantee:
      - 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
      - We guarantee our products work in the tested species and applications stated on the datasheet.
      - We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
      - We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

      Please note these conditions to our Abpromise®:
      - Protocol information must be provided in order for the claim to be validated.
      - Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
      - “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
      - Fast Track antibodies are covered for ELISA against the immunizing peptide only.

      https://www.abcam.com/abpromise

      I hope this information is helpful, but please do not hesitate to contact us with further questions.

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