Overview

  • Product name

    Anti-beta Endorphin antibody [B 31.15]
    See all beta Endorphin primary antibodies
  • Description

    Mouse monoclonal [B 31.15] to beta Endorphin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Human, Macaque monkey
  • Immunogen

    Full length native human beta Endorphin.

Properties

Applications

Our Abpromise guarantee covers the use of ab54205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance

    Beta-endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. ß-endorphin is a peptide, 31 amino acids long, resulting from processing of the precursor pro-opiomelanocortin (POMC). ß-endorphin is found in neurons of the hypothalamus as well as the pituitary gland. It is an agonist of the opioid receptors, with evidence suggesting that it serves as the endogenous ligand of the µ-opioid receptor, the same receptor to which the chemicals extracted from opium, such as morphine and codeine, have their analgesic and addictive effects
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Beta endorphin antibody
    • Corticotropin lipotropin antibody
    • POMC antibody
    • Proopiomelanocortin antibody

Images

  • ab54205, staining beta Endorphin in anterior (upper) and intermediate (lower) lobes of Wistar rat pituitary gland tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% normal horse serum in PBS for 30 minutes. Samples were incubated with primary antibody (1/2000 in diluent) overnight at 4°C.

References

This product has been referenced in:

  • Ranganathan S  et al. Molecular interpretation of ACTH-ß-endorphin coaggregation: relevance to secretory granule biogenesis. PLoS One 7:e31924 (2012). IHC-Fr ; Rat . Read more (PubMed: 22403619) »
  • Ubuka T  et al. Gonadotropin-inhibitory hormone identification, cDNA cloning, and distribution in rhesus macaque brain. J Comp Neurol 517:841-55 (2009). ICC/IF ; Macaque monkey . Read more (PubMed: 19844991) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Discount code: ******** I am very pleased to hear you would like to accept our offer and test ab54205 on rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat data and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Read More

Answer

Thank you for your question about our beta Endorphin antibody, ab54205. We have the same clone, B31.15, listed under a different product identifier, ab1419. The title of a reference listed on the datasheet of that product suggests that the clone will recognize POMC, but I have not confirmed. beta Endorphin antibody [B31.15] (ab1419) Click here (or use the following: https://www.abcam.com/index.html?datasheet=1419). Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage. Andjelkov N et al. Cell Biochem Funct. 2006 May-Jun;24(3):229-35. http://www.ncbi.nlm.nih.gov/pubmed/15898126?dopt=Abstract The antibody is raised against beta endorphin, but it is not clear if the epitope that it recognizes is unique to beta enodrphin, or is also present on POMC. I hope the reference is useful. If you have any questions, please contact us.

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