Anti-beta Galactosidase antibody (ab12081)

Reviews (1)Q&A (3)References (6)
Western blot - Anti-beta Galactosidase antibody (ab12081)

    Key features and details

    • Goat polyclonal to beta Galactosidase
    • Suitable for: WB
    • Reacts with: Escherichia coli
    • Isotype: IgG

    Overview

    • Product name

      Anti-beta Galactosidase antibody
      See all beta Galactosidase primary antibodies

    • Description

      Goat polyclonal to beta Galactosidase

    • Host species

      Goat

    • Specificity

      Detects recombinant beta galactosidase enzyme by Western blotting.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Escherichia coli

    • Immunogen

      Recombinant full length protein corresponding to Escherichia coli beta Galactosidase.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.025% Sodium azide
      Constituent: PBS

      This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab12081 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.

    Target

    • Relevance

      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      Images

      • Western blot - Anti-beta Galactosidase antibody (ab12081)
        Western blot - Anti-beta Galactosidase antibody (ab12081)
        Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

        Secondary
        Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size: 116 kDa
        Observed band size: 120 kDa
        why is the actual band size different from the predicted?


        Exposure time: 90 seconds

      References (6)

      Publishing research using ab12081? Please let us know so that we can cite the reference in this datasheet.

      ab12081 has been referenced in 6 publications.

      • Gong X  et al. Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib. Cancer Cell 32:761-776.e6 (2017). PubMed: 29232554
      • Brzoska E  et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). ICC . PubMed: 25890097
      • Mojiri A  et al. Hypoxia results in upregulation and de novo activation of von Willebrand factor expression in lung endothelial cells. Arterioscler Thromb Vasc Biol 33:1329-38 (2013). PubMed: 23580145
      • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . PubMed: 23223527
      • Zimmer C  et al. Role of Fgf8 signalling in the specification of rostral Cajal-Retzius cells. Development 137:293-302 (2010). IHC-Fr ; Mouse . PubMed: 20040495
      • Liebner S  et al. Wnt/beta-catenin signaling controls development of the blood-brain barrier. J Cell Biol 183:409-17 (2008). IHC-Fr . PubMed: 18955553

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-beta Galactosidase antibody

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
      Loading amount
      20 µg
      Specification
      bact beta-gal protein expressed in rat epithelial
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Dr. Silvia Finnemann

      Verified customer

      Submitted Aug 13 2012

      Question

      Inquiry: Hello. I just received a shipment of ab12081 Goat anti LacZ lot. As I was aliquoting the antibody for long term storage at -20 I only was able to retrieve 70uL of antibody but there should be 100ul in the vial. The vial was centrifuged before opening and the inside of the lid was checked for residual liquid.. none was found. Also the antibody solution had a noticeable cloudy white appearance that I have not noticed in other lots of this product before. Thanks in advance for your assistance. Cheers Steve

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience this has caused. I have issued a free of charge replacement for a full vial of ab12081 with the order number xxxxx. As to the cloudy appearance I would recommend spinning the antibody down for a few minutes and drawing the antibody from the top. You should not see any reduction in affinity. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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      Question

      My Fluorescent signal is weak using xylene based mounting media.

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      Answer

      Thank you for your phone call.

      As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with DAPI. I hope this heps, if not, please let me know and I will be happy to assist you further.

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      Question

      Thanks for your help on this matter. Much appreciated.  I have attached an email showing the purchase of this anti lacZ antibody from March 24 2011... just short of 6 months.  The current lot # we are using is 134764. Would you happen to know if there is a different lot to try?   Also I was hoping to just place the order through Cedarlane again if that is OK with you?  Thanks again

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      Answer

      Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your reply.

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