Product nameAnti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies
DescriptionGoat polyclonal to beta Galactosidase
SpecificityDetects recombinant beta galactosidase enzyme by Western blotting.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Escherichia coli
Recombinant full length protein corresponding to Escherichia coli beta Galactosidase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.025% Sodium azide
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab12081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.|
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
This product has been referenced in:
- Gong X et al. Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib. Cancer Cell 32:761-776.e6 (2017). Read more (PubMed: 29232554) »
- Brzoska E et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). ICC . Read more (PubMed: 25890097) »