Overview

  • Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Goat polyclonal to beta Galactosidase
  • Host species
    Goat
  • Specificity
    Detects recombinant beta galactosidase enzyme by Western blotting.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein of Escherichia coli beta Galactosidase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.025% Sodium azide
    Constituent: PBS

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.

Target

  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Images

    • Western blot - Anti-beta Galactosidase antibody (ab12081)
      Western blot - Anti-beta Galactosidase antibody (ab12081)
      Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 116 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)


      Exposure time: 90 seconds

    References

    This product has been referenced in:
    • Brzoska E  et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). ICC . Read more (PubMed: 25890097) »
    • Mojiri A  et al. Hypoxia results in upregulation and de novo activation of von Willebrand factor expression in lung endothelial cells. Arterioscler Thromb Vasc Biol 33:1329-38 (2013). Read more (PubMed: 23580145) »

    See all 5 Publications for this product

    Publishing research using ab12081? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-beta Galactosidase antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
    Loading amount
    20 µg
    Specification
    bact beta-gal protein expressed in rat epithelial
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    Username

    Dr. Silvia Finnemann

    Verified customer

    Submitted Aug 13 2012

    Inquiry: Hello. I just received a shipment of ab12081 Goat anti LacZ lot. As I was aliquoting the antibody for long term storage at -20 I only was able to retrieve 70uL of antibody but there should be 100ul in the vial. The vial was centrifuged before ...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience...

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    My Fluorescent signal is weak using xylene based mounting media.

    Thank you for your phone call.

    As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with...

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    Thanks for your help on this matter. Much appreciated.  I have attached an email showing the purchase of this anti lacZ antibody from March 24 2011... just short of 6 months.  The current lot # we are using is 134764. Would you happen to ...

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    Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your re...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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