Anti-beta Galactosidase antibody (ab12081)
Key features and details
- Goat polyclonal to beta Galactosidase
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Goat polyclonal to beta Galactosidase -
Host species
Goat -
Specificity
Detects recombinant beta galactosidase enzyme by Western blotting. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli beta Galactosidase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa. |
Target
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Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Images
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Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
Datasheets and documents
References (6)
ab12081 has been referenced in 6 publications.
- Gong X et al. Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib. Cancer Cell 32:761-776.e6 (2017). PubMed: 29232554
- Brzoska E et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). ICC . PubMed: 25890097
- Mojiri A et al. Hypoxia results in upregulation and de novo activation of von Willebrand factor expression in lung endothelial cells. Arterioscler Thromb Vasc Biol 33:1329-38 (2013). PubMed: 23580145
- Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . PubMed: 23223527
- Zimmer C et al. Role of Fgf8 signalling in the specification of rostral Cajal-Retzius cells. Development 137:293-302 (2010). IHC-Fr ; Mouse . PubMed: 20040495
- Liebner S et al. Wnt/beta-catenin signaling controls development of the blood-brain barrier. J Cell Biol 183:409-17 (2008). IHC-Fr . PubMed: 18955553