Anti-beta Galactosidase antibody (ab134435)
Key features and details
- Chicken polyclonal to beta Galactosidase
- Suitable for: IHC-FoFr, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgY
Overview
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Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Chicken polyclonal to beta Galactosidase -
Host species
Chicken -
Tested applications
Suitable for: IHC-FoFr, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Purified beta Galactosidase (lacZ) protein emulsified in Freund's adjuvants.
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Positive control
- Tissues infected with a virus bearing the LacZ gene
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General notes
Do not freeze these antibodies at –80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
IgY fractions were prepared from eggs yolks, and then affinity-purified antibodies were prepared using beta Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene.
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ICC/IF |
1/2500 - 1/5000. Use 2%
paraformaldehyde-fixed cells.
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Notes |
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IHC-FoFr
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene. |
ICC/IF
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
Target
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Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Images
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Immunohistochemical analysis of tissue infected with a virus bearing the LacZ gene labelling beta Galactosidase with ab134435 at 1/5000 dilution with e18 mouse otic vesicle. The green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab134435 has been referenced in 11 publications.
- Siddall NA et al. MiMIC analysis reveals an isoform specific role for Drosophila Musashi in follicle stem cell maintenance and escort cell function. Cell Death Discov 8:455 (2022). PubMed: 36371343
- Kim Y & Cho KO POU domain motif3 (Pdm3) induces wingless (wg) transcription and is essential for development of larval neuromuscular junctions in Drosophila. Sci Rep 10:517 (2020). PubMed: 31949274
- Jiang Z et al. MT1-MMP deficiency leads to defective ependymal cell maturation, impaired ciliogenesis, and hydrocephalus. JCI Insight 5:N/A (2020). PubMed: 32229724
- Spannl S et al. Glycolysis regulates Hedgehog signalling via the plasma membrane potential. EMBO J 39:e101767 (2020). PubMed: 33021744
- Ricolo D & Araujo SJ Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching. Elife 9:N/A (2020). PubMed: 33112231