Overview

  • Product name

    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description

    Chicken polyclonal to beta Galactosidase
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-FoFr, ICC/IFmore details
  • Immunogen

    Purified beta Galactosidase (lacZ) protein emulsified in Freund's adjuvants.

  • Positive control

    • Tissues infected with a virus bearing the LacZ gene
  • General notes

    Do not freeze these antibodies at –80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    IgY fractions were prepared from eggs yolks, and then affinity-purified antibodies were prepared using beta Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/2500 - 1/5000. Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene.
ICC/IF 1/2500 - 1/5000. Use 2% paraformaldehyde-fixed cells.

Target

  • Relevance

    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Images

  • Immunohistochemical analysis of tissue infected with a virus bearing the LacZ gene labelling beta Galactosidase with ab134435 at 1/5000 dilution with e18 mouse otic vesicle. The green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).

References

This product has been referenced in:

  • Lee J  et al. Vaccinia-related kinase 2 plays a critical role in microglia-mediated synapse elimination during neurodevelopment. Glia N/A:N/A (2019). Read more (PubMed: 31050055) »
  • Rueda N  et al. Anti-IL17 treatment ameliorates Down syndrome phenotypes in mice. Brain Behav Immun N/A:N/A (2018). Read more (PubMed: 29758264) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain

Dr. Antonio Romero Ruiz

Verified customer

Submitted Apr 04 2016

Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (Whole embryos. Formeldahyde fixed.)
Specification
Whole embryos. Formeldahyde fixed.

Abcam user community

Verified customer

Submitted Jan 27 2016

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Heart section in OCT)
Specification
Heart section in OCT

Alvaro Sahun

Verified customer

Submitted Jan 22 2016

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with ab134435.

To check the status of the order please contact our Customer Service team and reference this number. We are showing a delivery of Dec 27th or 28th.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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