Key features and details
- Rabbit polyclonal to beta Galactosidase
- Suitable for: WB, IP, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
Product nameAnti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies
DescriptionRabbit polyclonal to beta Galactosidase
SpecificityThis antibody recognizes beta Galactosidase from E coli and beta Galactosidase tagged proteins.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Escherichia coli
Recombinant full length protein corresponding to Escherichia coli beta Galactosidase aa 1-1024.
MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ QLRSLNGEWQ FVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDA PIYTNVTYPITVNPPFVPAENP TGCYSLTFNIDECWLQEGQTRIIFDG VNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRA GKNRLAVMVLRWSD GSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAV LE AEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTL RLNVENPA LWSAEIPNIYRAVVELHTADGTLIEAEACDVGFREVRIEN GLLLLNGKPLLIRGVNRHEH HPLHGQVMDEQTMVQDILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPA MSERVTRMVLRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVD PSRP VQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGELRPL ILCEYA HAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDEN GNPWSAYGGDFGDTPNDR QFCMNGLVFADRTPHPALTEAKHQQQFFQF RLSGRTIEVTSEYLFRHSDNELLHWTVALD GKPLASGEVPLDVAPQGK QVIELPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQ WRLAEN LSVTLPTASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDEKQ LLTP LRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALL QCTADTLADAVLITTA HAWQHQGKTLFISRKTYRIDGSGQMAITVDVE VASDTPHPARIGLTCQLAQVAERVNWLG LGPQENYPDRLTAACFDRWD LPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNI SRYSQQQL METSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQ LV WCQK
Database link: B7UJI9
- Purified beta Galactosidase; IPTG induced E.coli K12 cell extract; HEK293A cells transfected with beta Galactosidase cDNA.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab221199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 116 kDa.|
|IP||1/200 - 1/500.|
|ICC/IF||1/200 - 1/500.|
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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Immunofluorescent analysis of HEK293A cells transfected with beta Galactosidase cDNA (4% PFA-fixed, methanol permeabilized) labeling beta Galactosidase with ab221199 at 1/500 dilution, followed by secondary detection with Alexa Fluor 555 conjugated anti rabbit IgG at 1/500 dilution (red). Hoecst 33342 was used for nuclear counterstaining (blue).
All lanes : Anti-beta Galactosidase antibody (ab221199) at 1/1000 dilution
Lane 1 : purified beta Galactosidase at 0.01 µg
Lane 2 : uninduced E coli cell extract at 30 µg
Lane 3 : ITPG induced E coli cell extract at 30 µg
Predicted band size: 116 kDa
beta Galactosidase was immunorecipitated from ITPG induced E coli crude extracts using ab221199 at 1/500 dilution.
Western blot detection was performed using ab221199 at 1/1000 dilution.
Lane 1: Crude extract
Lane 2: Supernatant of the immunoprecipitated E. coli crude extract
Lane 3: Immunoprecipitate of crude E. coli extract
ab221199 has not yet been referenced specifically in any publications.