- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
DescriptionRabbit polyclonal to beta Galactosidase
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Host speciesRabbit
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SpecificityDetects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
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Tested applicationsSuitable for: IHC-P, WBmore details
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Immunogen
Full length native protein (purified) (E. coli).
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Positive control
- This antibody detects beta galactosidase in HeLa cells in WB
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityIgG fraction
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab4761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 17268532 | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa). |
Target
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RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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Cellular localizationCytoplasmic
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Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Images
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Western blot - Anti-beta Galactosidase antibody (ab4761)All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution
Lane 1 : Beta Gal recombinant protein at 0.1 µg
Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDaWestern blot using ab4761 at 1/1000.
Lane 1: 0.1µ
Lane 2: 20µ µ
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (1/5000).
Expected size: 116kD
References
This product has been referenced in:
- Clemente C et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). Read more (PubMed: 29500407) »
- Blanco MJ et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). Read more (PubMed: 28926609) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"