Product nameAnti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies
DescriptionRabbit polyclonal to beta Galactosidase
SpecificityDetects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Escherichia coli
Full length native protein (purified) (E. coli).
- This antibody detects beta galactosidase in HeLa cells in WB
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Our Abpromise guarantee covers the use of ab4761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 17268532|
|WB||Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).|
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution
Lane 1 : Beta Gal recombinant protein at 0.1 µg
Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Western blot using ab4761 at 1/1000.
Lane 1: 0.1
g Beta Gal recombinant protein µ
Lane 2: 20
g HeLa WCL + 0.1 µ g Beta Gal recombinant protein µ
Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (1/5000).
Expected size: 116kD
This product has been referenced in:
- Clemente C et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). Read more (PubMed: 29500407) »
- Blanco MJ et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). Read more (PubMed: 28926609) »