Anti-beta Galactosidase antibody (ab4761)

Reviews (1)Q&A (9)References (10)

Overview

  • Product name

    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies

  • Description

    Rabbit polyclonal to beta Galactosidase

  • Host species

    Rabbit

  • Specificity

    Detects recombinant beta Galactosidase in Western blotting. When used in Western blotting on 20ug Hela cell lysate dosed with 0.1ug of beta Galactosidase, detects a single strong band corresponding to beta Galactosidase.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Full length native protein (purified) corresponding to Escherichia coli beta Galactosidase.

  • Positive control

    • This antibody detects beta galactosidase in HeLa cells in WB

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 17268532
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).

Target

  • Relevance

    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Images

    • Western blot - Anti-beta Galactosidase antibody (ab4761)
      Western blot - Anti-beta Galactosidase antibody (ab4761)
      All lanes : Anti-beta Galactosidase antibody (ab4761) at 1/1000 dilution

      Lane 1 : Beta Gal recombinant protein at 0.1 µg
      Lane 2 : 20ug HeLa WCL + 0.1ug Beta Gal recombinant protein at 20 µg

      Secondary
      All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 116 kDa
      Observed band size: 116 kDa



      Western blot using ab4761 at 1/1000.

      Lane 1: 0.1µg Beta Gal recombinant protein
      Lane 2: 20µg HeLa WCL + 0.1µg Beta Gal recombinant protein

      Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (1/5000).
      Expected size: 116kD

    References

    This product has been referenced in:

    See all 10 Publications for this product

    Publishing research using ab4761? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Galactosidase antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (colon)
    Specification
    colon
    Fixative
    formalin
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH=6
    Permeabilization
    No
    Blocking step
    2%BSA, 10% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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    Abcam user community

    Verified customer

    Submitted Jan 10 2013

    Question

    Bonjour,

    De notre côté, c'est bon pour tester l'anticorps Ab4761 sur coupes paraffines de souris et de partager nos résultats. Comment faire pour passer la commande ?

    Cordialement,

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    Answer

    Code de remise : xxxxxx
    Date d’expiration : xxxxxxxx
    Valeur: €xxxxxx

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab4761 chez la souris. Le code de réduction est xxxxxxxxxx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre produit.

    Premièrement pour passer la commande vous devez soit téléphoner à notre service clientèle sur : 01.46.94.62.96 ou envoyer votre commande par Email à mailto:orders@abcam.com ou par Fax au 01.70.80.96.08.

    Une fois que vous avez testé l’anticorps, vous devez soumettre une Abreview avec vos résultats chez la souris en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab4761.

    Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

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    Question

    Sur quelles espèces a-t-il déjà été testé ? en IHC-P ?? et pour les autres techniques ??

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    Answer

    Merci de nous avoir contactés.

    L’anticorps ab4761 a été testé en IHC-P et WB. L’espèce testée en IHC-P est l’humain.

    Voir la publication suivante : Hachiya A et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther 14:648-56 (2007). Pour en lire plus allez voir sur PubMed: 17268532

    L’anticorps a aussi été testé en WB avec des lysâtes de cellules Hela.

    Si vous ne préférez pas essayer cet anticorps chez la souris et de participer à notre offre de test, vous pouvez essayer l’anticorps ab9361 (www.abcam.com/ab9361) qui lui a été testé chez la souris en IHC-P et est couvert par notre Garantie Abpromise.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Question

    Désolée de revenir encore une fois vers vous mais il y a vraiment très peu d'informations concernant l'utilisation de l'anticorps Ab4761 (anti-betagalactosidase) pour immunohistochimie en paraffine.

    Est il censé marcher sur coupes de souris fixées Formalin ???

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    Answer

    Merci de nous avoir contactés.

    Nous n'avons pas encore testé l’anticorps ab4761 chez la souris. Cependant, puisque nous n'avons pour l'instant pas d'image IHC-P chez la souris à mettre sur la fiche technique du ab4761 il me sera possible de vous offrir une remise si vous acceptez de tester ab4761 chez la souris et de partager vos résultats. Après avoir soumis vos résultats sous forme d'Abreview, un avoir de la valeur de l'anticorps ab4761 sera alors crédité sur votre compte client.

    Étapes à suivre :

    1. Nous informer que vous souhaitez tester l’anticorps chez la souris et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,

    2. Acheter l'anticorps,

    3. Nous envoyer vos résultats (positifs ou négatifs) obtenus sous forme d'Abreview (www.abcam.com/abreviews).

    4. L'envoi de vos résultats activera le code et vous donnera un avoir de la valeur de l'anticorps testé.

    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

    Sinon, je peux vous proposer d’utiliser un autre anticorps ab9361 (https://www.abcam.com/ab9361) qui a été testé sur coupes de souris fixées Formalin en IHC-P et est couvert par notre Garantie Abpromise. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique (espèces et applications), nous offrons un remboursement ou un remplacement gratuit (https://www.abcam.com/abpromise).

    Cet anticorps est un polyclonal de poulet. Nous avons des anticorps anti-poulet secondaires si cela vous intéresse :

    Goat anti-Chicken IgY H&L (DyLight® 488) secondary antibody (ab96947)
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 550) (ab96948)
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 594) (ab96949)
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650) (ab96950)




    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Question

    Product code: 4761

    Inquiry: Dear customer service, I am really confused about this product. People are asking you if it could be suitable on human tissue sections and your answer is yes. Does it mean that this antibody cross reacts with human b-galactosidase? If so, does it also cross react with murine b-galactosidase? My understanding is that the immunogen is the E.coli form, therefore -unless the epitope is conserved between human and bacteria, it should not cross react. Many thanks for your reply

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    Answer

    Thank you for your inquiry.
    I can confirm that ab4761 will detect beta Galactosidase from E.coli origin.
    If beta Galactosidase is overexpressed (ectopically expressed) in human tissue than this antibody is suitable for this.
    We tested this antibody with beta Galactosidase that was expressed in HeLa cells.
    I hope this information is helpful and apologize for the confusion. Please feel free to contact me again if you have any further questions.

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    Question

    Hi there,
    answers to the questionnaire as below:
    1) Abcam product code: ab4761

    2) Abcam order reference number or product batch number: TZ141959
    3) Description of the problem: No staining achieved
    4) Sample preparation:
    Species: Human
    Type of sample: PFA/formalin fixed paraffin embedded sections
    Positive control: Internal positive control using human lung tissue sections
    Negative control: IgG isotype control
    5) Fixation step
    Yes
    If yes: Formalin 10%
    Fixation time: unknown
    Fixation temperature: room temperature
    6) Antigen retrieval method
    1 minute pressure cooker in citrate buffer
    5 minutes pressure cooker in citrate buffer
    10 minutes 100% microwave power in citrate buffer
    20 minutes 100% microwave power in citrate buffer
    5 minutes pressure cooker in EDTA
    10 minutes 100% microwave power in EDTA
    20 minutes 100% microwave power in EDTA
    7) Permeabilization method:
    None
    8) Blocking agent (eg BSA, serum…):
    None

    9) Endogenous peroxidases blocked?
    Yes in 0.3% H2O2
    10) Primary antibody (If more than one was used, describe in “additional notes”) :
    1/100 in 3% BSA (TBS buffer) overnight at 4 degrees
    11) Secondary antibody: Dako EnVision+ System-HRP
    Species: Goat
    Reacts against: Rabbit
    Concentration or dilution: RTU
    Diluent buffer: Tris-HCL
    Incubation time: 30 minutes
    Enzyme conjugated (peroxidase labeled polymer conjugated to goat anti-rabbit)
    12) Washing after primary and secondary antibodies:
    Buffer: TBS
    Number of washes: 2 x 5 min washes following primary incubation and 1 x 5 min wash following secondary incubation
    13) Detection method
    DAB
    14) How many times have you run this staining? 7
    Do you obtain the same results every time? Yes
    What steps have you altered to try and optimize the use of this antibody? Retrieval times and retrieval buffers used.
    Images of the different retrievals tested are attached.
    Kind regards,

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    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
    I would like to reassure you that ab4761 is tested and covered by our 6 month guarantee for use in samples that overexpress beta Galactosidase and IHC. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
    Reviewing this case, I would like to offer some suggestions to help optimise the results from ab4761.
    1.) I recommend to use a permeabilization step since I assume that the beta Galactosidase will be overexpressed in the cytoplasm. 0.1% triton for 10 minutes only at RT.
    2.) The BSA in the primary antibody solution can be omitted.
    3.) I recommend to run a WB with the sample to confirm the expression of beta Galactosidase.
    4.) It might be worth to try without antigen retrieval.
    I would also appreciate if you can confirm some further details: 1.) Please give more details where the beta Galactosidase is overexpressed, what system was used and how the expression was tested.
    2.) Is the secondary antibody and the rest of the detection reagents working?
    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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    Question

    Thank you for your reply. I would like to verify I understood you correctly. We buy ab4761 for the full price (£234), test it, submit abreview and then get
    £234 discount for any Ab from Abcam. Correct?



    Thank you,

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    Answer

    Thank you for your reply.

    It is absolutely correct that you will get the full price of ab4761 as a discount off a future order.

    If you would like to take part in this program, please let me know. I will be happy to issue a testing discount code for you that you will need to add to the additional notes sectionwhen submitting the Abreview and, of course, when you want to use the discount after submitting the Abreview.

    I am looking forward to your reply.

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    Question

    Dear Sir or Madame,

    I am a graduate student at Scottish Centre of Regenerative Medicine.

    We are interested in unconjugated rabbit or rat anti-beta-galactosidase antibody (anti-LacZ Ab). We plan to use this Ab on mouse embryonic tissues:routinelyfor whole mount staining andoccasionallyfor cryo-sections. We have found several anti-LacZ Abs on your web-site, but they seems are not recommended for our particular application.

    Those are ab616 and ab4761. ab616 is not recommended for immunohistochemistry according to yourdescription. The only option left is ab4761, however it was tested with parafin sections and WB; and there are no images. I have heard (on "Origins of tissue stem cells" conference organized with Abcam, June 29, 2012) that in such circumstances we can buy the antibody with some discount. Could you advise, please.

    Sincerely,

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    Answer

    Thank you very much for your interest in ab4761 and ab616.

    I confirm that we cannot guarantee ab616 for IHC and we also do not recommend this antibody for this application.

    To our knowledge, ab4761 has not been tested in IHC - Wholemount. Therefore, I can offer a discount off a future purchase if you buy ab4761 now, test it in IHC - Wholemountand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab4761 off a future order.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab4761 in IHC - Wholemount. I will then send a discount code. This code must be issued before purchasing ab4761 so please wait for my reply before ordering.

    2. Purchase ab4761 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC - Wholemount.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab4761 turns out to be unsuitable for IHC - Wholemount, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Question

    Hi,
    I'm looking to use the beta galactosidase polyclonal antibody (ab4761) for FFPE IHC on human lung tissue.
    Has this product been successfully used on human tissue via IHC for paraffin embedded sections?
    If this is unknown would it be possible to get a sample of the antibody
    before purchase or a reduction in cost if the antibody is unsuccessful?
    Kind regards,

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    Answer

    Thank you for your inquiry.
    I am happy to confirm that ab4761 is tested and guaranteed for IHC-P.
    The following publication even used this antibody with human/ viral transfected samples:
    Hachiya A et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther 14:648-56 (2007).
    I attached the publication for more information on the protocol.
    Because we carry over 75,000 products, it isn't feasible for us to keep small sample sizes of our products.
    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.
    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
    To find out more about our Abreview system, please see the following link:
    https://www.abcam.com/abreviews
    I hope this information is helpful and wish you good luck with your research.

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    Question

    Good afternoon

    I wonder if you can give me some info on the antibodies you have available.

    I want to demonstrate Beta Galactosidase in paraffin sections of human lung tissue.

    Do you have any antibody which would be suitable for this application

    Thanks for your help

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    Answer

    Thank you for contacting us.

    We have several antibodies against beta galactosidase, two of which have been validated for IHC staining of paraffin embedded sections. You may want to consider chicken polyclonal ab9361 or rabbit polyclonal ab4761. These have not been validated in human tissue specifically but should be compatible in detecting the Beta galactosidase protein.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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