Overview

  • Product name
    Anti-beta Galactosidase antibody
    See all beta Galactosidase primary antibodies
  • Description
    Rabbit polyclonal to beta Galactosidase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IPmore details
    Unsuitable for: ICC/IF,IHC-FoFr,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant full length protein (E. coli).

  • Positive control
    • 1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
  • General notes

    Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.

sELISA tested against 1ug E.coli beta galactosidase.

WB 1/5000 - 1/10000. Detects a band of approximately 116 kDa.

This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives.

IP 1/500 - 1/5000.
  • Application notes
    Is unsuitable for ICC/IF,IHC-FoFr,IHC-Fr or IHC-P.
  • Target

    • Relevance
      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • Cellular localization
      Cytoplasmic
    • Database links
      • Alternative names
        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      Images

      • Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

      References

      This product has been referenced in:
      • Chen ZJ  et al. Renoprotective Effect of a Chinese Herbal Formula, Qidan Dihuang Decoction, on Streptozotocin-Induced Diabetes in Rat. Evid Based Complement Alternat Med 2018:7321086 (2018). Read more (PubMed: 29849726) »
      • Chodelkova O  et al. Tcf7L2 is essential for neurogenesis in the developing mouse neocortex. Neural Dev 13:8 (2018). IHC-P . Read more (PubMed: 29751817) »
      See all 96 Publications for this product

      Customer reviews and Q&As

      1-10 of 74 Abreviews or Q&A

      Answer

      I am very pleased to hear you would like to accept our offer and testab616 and ab15580in ICE. These codes will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for ICE for each antibody and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Western blot

      Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (epidermis)
      Specification
      epidermis
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

      Abcam user community

      Verified customer

      Submitted May 29 2013

      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate
      Sample
      Mouse Tissue sections (epidermis)
      Specification
      epidermis
      Permeabilization
      No
      Fixative
      Paraformaldehyde

      Abcam user community

      Verified customer

      Submitted May 28 2013

      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (ROSA LacZ/+ AMH-Cre adult testes)
      Specification
      ROSA LacZ/+ AMH-Cre adult testes
      Fixative
      4 ? Acetone:Methanol 1:1
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

      Dr. Qing Wen

      Verified customer

      Submitted Dec 20 2012

      Answer

      Merci de nous avoir contactés.

      J'ai pu voir d'après nos archives que plusieurs utilisateurs ont rencontré des problèmes avec cet anticorps en IHC-P. L'application IHC-P a d'ailleurs été retirée de la liste des applications testées, mais malheureusement pas l'image que vous avez mentionnée; ceci est une erreur de notre part que je vais signaler à mes collègues responsables des fiches techniques.

      De plus, nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, je peux vous recommander d’utiliser l’anticorps ab4761 qui a été testé en IHC-P et est couvert par notre Garantie Abpromise. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique (espèces et applications), nous offrons un remboursement ou un remplacement gratuit (https://www.abcam.com/abpromise).


      J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

      Read More

      Answer

      Thank you for submitting an Abreview for ab616. As you may have noticed, your review has now been published on our website.
      Since you obtained poor results using the antibody in an untested species, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
      Based on the information in your review, I have a few more questions:
      1) Did you use the antibody to detect human beta Galactosidase ?
      2) If not, for which application or species was the discount code given?
      In terms of suggestions:
      - It might be helpful to compare the resuts obtained with human beta Galactosidase with E.coli beta Galactosidase.
      If you decide to follow these suggestions, please let me know if they are helpful.
      I wish you good luck with your research.

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      ELISA
      Sample
      Human Cell (H407 cells)
      Specification
      H407 cells
      Blocking step
      AbCam ICE kit as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
      Type
      Indirect

      Abcam user community

      Verified customer

      Submitted Oct 10 2012

      Answer

      Thank you for contacting us yesterday.

      I am sorry to hear you are experiencing difficulties with one of our products. As discussed over the phone, I'd like to try and help you to improve the results currently obtained. In order to do this, could you please fill out the questionnair I have attached to this email. If you could also include some images of the blots obtained that would be very helpful. I understand that you have already performed a lotof optimisation but if you could just briefly outline what you have tried to optimise that would be very helpful.

      I think it would be worthwhile to try and use the beta Galactosidase protein (ab7981) which you have just to check that there is not a problem with the antibody itself thatare contributing to the poor results.

      In answer to you questions in regards to how to use this protein as a control. We recommend reconstituting the protein in a minimum concentration of 1 mg/mL in 0.1 M Sodium/potassium phosphate buffer, pH 7.0. This can be performed as follows:

      0.1 M Sodium phosphate buffer: Dissolve 1.42 gms sodium phosphate dibasic (MW 141.98) in reagent grade water to a final volume of 100 ml.

      0.1 M Potassium phosphate buffer: Prepare by dissolving 1.36 gms potassium phosphate monobasic (MW 136.1) in reagent grade water to a final volume of 100 ml.

      0.1 M Sodium/potassium phosphate, pH 7.0: Take 60 ml sodium phosphate buffer 0.1 M and adjust pH to 7.0 with 0.1 M potassium phosphate buffer. Buffer may be stored at 2 - 8°C for 2 - 3 weeks.

      The lypolised powder the protein is provided as can be stored at2-8°C but once dissolved it is best to aliquot and freeze stocks at -20C or -80C. We do not have any reconstituted stability data andwould therefore recommend to reconstitute the amount needed for the assay fresh just prior to performing the assay.

      I would recommend initially loading 0.1 ug in a well to see how well the antibody performs next to your samples. This value may need to be reduced if the signal observed is too strong compared to your lysates.

      I hope this information has been of help. I look forward to receiving your reply.

      Read More

      Answer

      Vielen Dank für Ihre Anfrage.

      Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird, können wir leider keinen Ersatz anbieten, wir möchten Sie jedoch einladen unser Abreview-System zu verwenden.

      Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.

      Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:

      https://www.abcam.com/abreviews

      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

      Read More

      1-10 of 74 Abreviews or Q&A

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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