Anti-beta Galactosidase antibody (ab9361)
Key features and details
- Chicken polyclonal to beta Galactosidase
- Suitable for: IHC-FrFl, IHC-FoFr, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Escherichia coli
- Isotype: IgY
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-beta Galactosidase antibody
See all beta Galactosidase primary antibodies -
Description
Chicken polyclonal to beta Galactosidase -
Host species
Chicken -
Tested applications
Suitable for: IHC-FrFl, IHC-FoFr, ICC/IF, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length native protein (purified). The immunogen was purified beta-galactosidase from Escherichia coli.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl | (4) |
Use at an assay dependent concentration.
|
| IHC-FoFr | (4) |
1/1000 - 1/2000.
|
| ICC/IF | (9) |
Use at an assay dependent concentration.
|
| IHC-Fr | (9) |
Use at an assay dependent concentration.
|
| IHC-P | (7) |
1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-FrFl
Use at an assay dependent concentration. |
|
IHC-FoFr
1/1000 - 1/2000. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
-
Alternative names
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
Images
-
Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)Image from PLoS One. 2013; 8(11): e77928, Fig 2, doi: 10.1371/journal.pone.0077928 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
P0-adult mice were euthanized and perfused with 4% paraformaldehyde in PBS (PF). Their spinal cords were then post-fixed for 30–60 mins in 4% PF at 4°C (P0) or at room temperature (adult). Spinal cords were rinsed and cryoprotected in 20% sucrose in PBS (4°C) prior to embedding in OCT (Tissue-Tek). Immunostaining of frozen spinal sections was performed by incubating 20 µm thick sections with primary antibodies, which were then detected using species-specific secondary antibodies conjugated with Cy2, Cy3 and Cy5 or FITC. ab9361 was used at 1:1000.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Laurel Bagliaab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)Image courtesy of Dr Sean Speese by Abreview.ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)Image from PLoS One. 2013; 8(11): e80801, Fig 1B, doi: 10.1371/journal.pone.0080801 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Ab9361 staining Beta galactosidase in Mouse thyroid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed 10% buffered formalin, cut into 3-4 micron slices, blocked with 10% normal goat serum and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with the following primary antibodies; anti-BrdU, anti-Sca1 (ab109211, 1∶600), anti-NKX2-1 and anti-β-gal (ab9361, 1∶1000). Sections were incubated with the first primary antibody (anti-Sca1) for 1 hour at room temperature. After washing with PBS, sections were incubated with the first secondary antibody (Alexa Fluor 555 goat anti-rabbit IgG) and washed with PBS. Sections were then incubated with normal serum (5% rabbit serum) from the same host species as the first primary antibodies for 1 hour at room temperature and washed with PBS. Sections were further incubated with an excess of unconjugated Fab antibody derived from the same host species as the primary antibody for 1 hour at room temperature and washed with PBS. The sections were finally incubated with the mixed second primary antibodies (anti-BrdU, anti-β-gal, anti-NKX2-1) overnight at 4°C, washed with PBS, and were incubated with the second secondary antibody (Alexa Fluor 488 goat anti-rat IgG, Dylight 650 goat anti-chicken IgY, Dylight 594 goat anti-rabbit IgG) for 1 hour at room temperature and washed with PBS. DAPI dye was used to stain the nuclei of cells.
-
Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Dr Steven Hughesab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Dr Deon Wolpowitzab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (475)
ab9361 has been referenced in 475 publications.
- Marneros AG Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron. Cell Rep 34:108616 (2021). PubMed: 33440155
- Chatzeli L et al. Comparing development and regeneration in the submandibular gland highlights distinct mechanisms. J Anat 238:1371-1385 (2021). PubMed: 33455001
- Jing J et al. Reciprocal interaction between mesenchymal stem cells and transit amplifying cells regulates tissue homeostasis. Elife 10:N/A (2021). PubMed: 33480845
- Liang X et al. The SNAG Domain of Insm1 Regulates Pancreatic Endocrine Cell Differentiation and Represses ß- to d-Cell Transdifferentiation. Diabetes 70:1084-1097 (2021). PubMed: 33547047
- Lowenstein ED et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268