Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E12.5 Embryo)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EGTA, pH=9, pressure cooker
Permeabilization
Yes - Saponi
Specification
E12.5 Embryo
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 0.1% BSA+ 0.3% triton
Dilution
1/600
Additional data
Additional Notes
The embryo used, is heterozygous for a B-gal knock-in to the Shh locus, so stains in a Sonic expression pattern. The photo on the left shows staining with the B-gal antibody within a gut tube. The panel on the right shows matching staining detected with the anti Shh antibody (ab86462).
Abcam user community
Verified customer
Submitted Feb 16 2016