Overview

  • Product name
    Anti-beta Galactosidase antibody (Biotin)
    See all beta Galactosidase primary antibodies
  • Description
    Rabbit polyclonal to beta Galactosidase (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length protein (E. coli).

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20799882
WB 1/2000 - 1/10000.
IHC-P 1/1000 - 1/5000.

Target

  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Images

    • Anti-beta Galactosidase antibody (Biotin) (ab6645) at 1/1000 dilution + Beta Galactosidase at 0.05 µg with Blocking buffer

      Secondary
      HRP streptavidin conjugate at 1/40000 dilution

    References

    This product has been referenced in:
    • Matsumoto K  et al. In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay). Sci Rep 7:12136 (2017). Read more (PubMed: 28939819) »
    • Herrera JJ  et al. Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury. J Neurotrauma 27:2067-76 (2010). IHC-Fr ; Rat . Read more (PubMed: 20799882) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    I would recommend ab6645. We do not know if this antibody has been used in formalin treated tissue, however it has been used with IHC and I would expect it to work with formalin treated tissue as it is a polyclonal and thus recognises multiple epitopes.

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    Answer

    This antibody has not yet been tested in Flow Cytometry, therefore we can't guarantee that it will work. For a protocol regarding the staining of red blood cells, please see the general FACS protocol on the Abcam website

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    Answer

    This antibody is biotinylated so there will be no need for a secondary antibody. You only require an ABC kit and chromogen. I recommend ab8621 for the ABC kit (Super ABC / Horseradish Peroxidase Basic Kit) and ab8184 (HRP Chromogen kit (DAB)) for the chromogen. I will personally e-mail you my antigen retrieval protocol. Try the pressure cooker method for starters.

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    Question
    Answer

    Yes!

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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