Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Clone numberDC1 4C7
Light chain typekappa
Our Abpromise guarantee covers the use of ab116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
All lanes : Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg
Lane 1 :
Recombinant Human GLB1/Beta-galactosidase protein (ab151890)
Lane 2 :
beta Galactosidase protein (ab79449)
Lysates/proteins at 0.1 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 4 minutes
This product has been referenced in:
- Smith SJ et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . Read more (PubMed: 23272238) »
- Nassiri M et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . Read more (PubMed: 20431063) »