Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Assay time: 30 min
- Sample type: Inhibitor compounds
Product nameBeta Galactosidase (beta-gal) Inhibitor Screening Kit (Fluorometric)
Sample typeInhibitor compounds
Assay typeEnzyme activity (quantitative)
Assay time0h 30m
Assay durationMultiple steps standard assay
In this Beta Galactosidase (beta-gal) Inhibitor Screening Kit (Fluorometric) (ab284514) (K827), β-Gal converts β-Gal substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm).
In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase.
The assay is simple, high-throughput adaptable and can be performed within 30 min.
This product is manufactured by BioVision, an Abcam company and was previously called K827 Beta Galactosidase (ß-Gal) Inhibitor Screening Kit (Fluorometric). K827-100 is the same size as the 100 test size of ab284514.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests beta-Galactosidase Purple cap 1 vial beta-Galactosidase Assay Buffer WM cap 1 x 25ml beta-Galactosidase Inhibitor Control Orange cap 1 vial beta-Galactosidase Substrate (in DMSO) Blue cap 1 x 200µl
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
- Beta D galactosidase
- Beta gal
- Beta galactosidase
ab284514 has not yet been referenced specifically in any publications.