• Product name
    beta Galactosidase Detection Kit
    See all beta galactosidase kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
  • Product overview

    The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.

    Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 250 tests
    Fixative Solution (1X) NM 1 x 125ml
    Staining Solution (1X) WM 1 x 125ml
    Staining Supplement (100X) Red 1 x 1.5ml
    X-Gal Green 1 x 150mg
  • Research areas
  • Relevance
    Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity.
  • Cellular localization
    Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes.
  • Alternative names
    • Acid beta galactosidase
    • Beta galactosidase 1
    • EBP
    • ELNR1
    • Galactosidase beta 1
    • GLB1
    • MPS4B
    see all


  • Gamma-radiation induced senescence in hFOB cells. The number of senescence-associated-β-galactosidase positive cells (left panel) cleary increases 72h after 8 Gy γ irradiation. Representative data was from one of a total of 3 indepent experiments.

    Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604



This product has been referenced in:
  • Li XH  et al. REDD1 Protects Osteoblast Cells from Gamma Radiation-Induced Premature Senescence. PLoS One 7:e36604 (2012). Human . Read more (PubMed: 22629318) »

See 1 Publication for this product

Customer reviews and Q&As

The staining procedure will remain the same for suspension cells except the cells would have to be in a micro-centrifuge tube while the washing (add PBS, spin down, remove buffer) and incubation steps are performed. Once done, the cells can be aliquote...

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The protocol calls for fixing tissues for a short period of time (10 - 15 min at room temperature). Stored samples may not have enough enzymatic activity to do this assay. Residual enzymatic activity also depends on how high the formalin concentration ...

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You can keep both reagents at 37°C until the precipitate dissolves.

Thank you for your inquiry.

I can confirm that we have optimized ab102534 for cells.

We believe it is possible to use this kit with frozen tissues, I am sorry to confirm that we do not know what optimizations will be necessary.
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Thank you for contacting us.

I have obtained the following information from the lab:

1) ab102534 cannot be used for senescence assay.
ab65351 is used to detect senescence-associated expression of β-galactosidase (SA-&beta...

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