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Beta Galactosidase Detection Kit (Fluorometric) (ab176721) uses the fluorogenic fluorescein digalactoside (FDG) galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates the strongly fluorescent fluorescein upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. FDG used in the kit is not fluorescent. The galactosidase induced cleavage of FDG gives fluorescein that has the spectra of Ex/Em = 490/515 nm, which can be detected with most fluorescence instruments equipped with a FITC filter set. The kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader or a fluorescence microscope. It might also be used for screening galactosidase inhibitors or inducers.
|Beta-mercaptoethanol||1 x 500µl|
|DMSO||1 x 500µl|
|Fluorescein di-β-D-Galactopyranoside (FDG)||1 vial|
|Lysis Buffer||1 x 25ml|
|Reaction Buffer||1 x 50ml|
|Stop Buffer||1 x 25ml|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"