Overview

  • Product name
    Beta Galactosidase Detection Kit (Fluorometric)
    See all beta galactosidase kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Sensitivity
    >= 0.3 mU/well
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Beta Galactosidase Detection Kit (Fluorometric) (ab176721) uses the fluorogenic fluorescein digalactoside (FDG) galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates the strongly fluorescent fluorescein upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. FDG used in the kit is not fluorescent. The galactosidase induced cleavage of FDG gives fluorescein that has the spectra of Ex/Em = 490/515 nm, which can be detected with most fluorescence instruments equipped with a FITC filter set. The kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader or a fluorescence microscope. It might also be used for screening galactosidase inhibitors or inducers.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    Beta-mercaptoethanol 1 x 500µl
    DMSO 1 x 500µl
    Fluorescein di-β-D-Galactopyranoside (FDG) 1 vial
    Lysis Buffer 1 x 25ml
    Reaction Buffer 1 x 50ml
    Stop Buffer 1 x 25ml
  • Research areas
  • Relevance
    Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity.
  • Cellular localization
    Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes.
  • Alternative names
    • Acid beta galactosidase
    • Beta galactosidase 1
    • EBP
    • ELNR1
    • Galactosidase beta 1
    • GLB1
    • MPS4B
    see all

Images

  • Beta Galactosidase dose response was measured with Beta Galactosidase Detection Kit (Fluorometric) (ab176721) in a black 96-well plate using a fluorescence microplate reader. As low as 0.3 mU Beta Galactosidase can be detected with 30 minutes incubation.

References

This product has been referenced in:
  • Chhunchha B  et al. Prdx6 retards senescence and restores trabecular meshwork cell health by regulating reactive oxygen species. Cell Death Discov 3:17060 (2017). Read more (PubMed: 28904819) »

See 1 Publication for this product

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