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beta Galactosidase Staining Kit (ab102534)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (5)References (2)

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Beta Galactosidase Detection Kit (ab102534)

    Key features and details

    • Assay type: Direct
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      beta Galactosidase Staining Kit
      See all beta galactosidase kits
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Direct
    • Product overview

      The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.

      Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.

      Visit our FAQs page for tips and troubleshooting.

    • Notes

      This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 250 tests
      Fixative Solution (1X) NM 1 x 125ml
      Staining Solution (1X) WM 1 x 125ml
      Staining Supplement (100X) Red 1 x 1.5ml
      X-Gal, chromogenic beta-galactosidase substrate Green 1 x 150mg
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • proliferation and senescence
    • Relevance

      Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity.
    • Cellular localization

      Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes.
    • Alternative names

      • Acid beta galactosidase
      • Beta galactosidase 1
      • EBP
      • ELNR1
      • Galactosidase beta 1
      • GLB1
      • MPS4B
      see all

    Associated products

      Images

      • Beta Galactosidase Detection Kit (ab102534)
        Beta Galactosidase Detection Kit (ab102534)Li XH et al., PLoS One, 7, e36604, 2012 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/
        Gamma-radiation induced senescence in hFOB cells. The number of senescence-associated-β-galactosidase positive cells (left panel) cleary increases 72h after 8 Gy γ irradiation. Representative data was from one of a total of 3 indepent experiments. Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab102534? Please let us know so that we can cite the reference in this datasheet.

      ab102534 has been referenced in 2 publications.

      • Saheera S  et al. Protective effect of antioxidant Tempol on cardiac stem cells in chronic pressure overload hypertrophy. Life Sci 222:88-93 (2019). PubMed: 30822425
      • Li XH  et al. REDD1 Protects Osteoblast Cells from Gamma Radiation-Induced Premature Senescence. PLoS One 7:e36604 (2012). Human . PubMed: 22629318

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Easy to use kit

      Good Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      Overall the kit is easy to use and produced results we're looking for. Gave 4 out 5 star because of precipitation of staining supplement (100x). The precipitates wouldn't complete dissolve even after warmed the solution vial at 60C over 20 minutes.

      Helen Shi

      Verified customer

      Submitted Nov 13 2019

      Question

      Protocol for suspension cells

      Read More

      Abcam community

      Verified customer

      Asked on Nov 21 2013

      Answer

      The staining procedure will remain the same for suspension cells except the cells would have to be in a micro-centrifuge tube while the washing (add PBS, spin down, remove buffer) and incubation steps are performed. Once done, the cells can be aliquoted onto a slide and can be visualized under a microscope.

      Read More

      Kevin Hanson

      Abcam Scientific Support

      Answered on Nov 21 2013

      Question

      Might this product work when used with mouse, formalin fixed (pH 7.4) samples?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 08 2013

      Answer

      The protocol calls for fixing tissues for a short period of time (10 - 15 min at room temperature). Stored samples may not have enough enzymatic activity to do this assay. Residual enzymatic activity also depends on how high the formalin concentration is. However, it is worth a try to see if it works. We have not done this assay with formalin fixed tissues. It is ideal to do the assay on fresh tissues.

      Read More

      Abcam Scientific Support

      Answered on Apr 08 2013

      Question

      I see precipitate in the staining solution and staining supplement after warming to room temp after storage in the refrigerator. The protocol suggests warming to dissolve precip. What temp and for how long do you suggest to warm these reagents?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 01 2013

      Answer

      You can keep both reagents at 37°C until the precipitate dissolves.

      Read More

      Abcam Scientific Support

      Answered on Apr 01 2013

      Question

      Hi



      I was wondering if you (or if you know if any of your customers) have used the beta Galactosidase Detection Kit (ab102534) on paraffin embedded tissue on slides? If so, does the protocol differ from that supplied for wells/plates of cells?

      Sorry, I mean Frozen tissue, not paraffin embedded


      Many thanks

      Read More

      Abcam community

      Verified customer

      Asked on Nov 05 2012

      Answer

      Thank you for your inquiry.

      I can confirm that we have optimized ab102534 for cells.

      We believe it is possible to use this kit with frozen tissues, I am sorry to confirm that we do not know what optimizations will be necessary.

      This will have to be determined by the end user.

      To our knowledge, ab102534 has not been tested with frozen tissue. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

      Simply follow these easy steps below to apply for our AbTrial Program:

      1. Reply to this email, letting us know you are interested in testing this product.

      2. Our scientists will email you an inactive personal discount code for the value of the product.

      3. Purchase and test the product at the regular price.

      4. Submit your results, including your discount code in the additional notes section of your Abreview.

      5. Once the Abreview is submitted, the discount code will become active.

      6. Apply your discount code on your next order to receive that value off.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at:

      https://www.abcam.com/abtrial.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2012

      Question

      1) Can ab102534 be used with senescent cells?
      2) How does the composition of the reagents between both kits (ab102534 and ab65351) differ, it at all?
      3) What is the pH of the fixative solution (for each kit)?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 06 2012

      Answer

      Thank you for contacting us.

      I have obtained the following information from the lab:

      1) ab102534 cannot be used for senescence assay.
      ab65351 is used to detect senescence-associated expression of β-galactosidase (SA-β-Gal) activity, whereas ab102534 will detect all β-Gal activity and is used mainly for transfected cells.

      2) The composition of the reagents is proprietary information, but the pH is the main difference. Unfortunately, we do not disclose the actual pH. I am very sorry about that.

      I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Apr 06 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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