beta Galactosidase Staining Kit (ab102534)

The staining procedure will remain the same for suspension cells except the cells would have to be in a micro-centrifuge tube while the washing (add PBS, spin down, remove buffer) and incubation steps are performed. Once done, the cells can be aliquoted onto a slide and can be visualized under a microscope.

The protocol calls for fixing tissues for a short period of time (10 - 15 min at room temperature). Stored samples may not have enough enzymatic activity to do this assay. Residual enzymatic activity also depends on how high the formalin concentration is. However, it is worth a try to see if it works. We have not done this assay with formalin fixed tissues. It is ideal to do the assay on fresh tissues.

You can keep both reagents at 37°C until the precipitate dissolves.

Thank you for your inquiry.

I can confirm that we have optimized ab102534 for cells.

We believe it is possible to use this kit with frozen tissues, I am sorry to confirm that we do not know what optimizations will be necessary.

This will have to be determined by the end user.

To our knowledge, ab102534 has not been tested with frozen tissue. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at:

https://www.abcam.com/abtrial.

Thank you for contacting us.

I have obtained the following information from the lab:

1) ab102534 cannot be used for senescence assay.
ab65351 is used to detect senescence-associated expression of β-galactosidase (SA-β-Gal) activity, whereas ab102534 will detect all β-Gal activity and is used mainly for transfected cells.

2) The composition of the reagents is proprietary information, but the pH is the main difference. Unfortunately, we do not disclose the actual pH. I am very sorry about that.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.