Product namebeta-Glucosidase Assay Kit
Range2 mU/ml - 250 mU/ml
Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity. beta-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests β-NPG Substrate 1 x 1ml Assay Buffer 1 x 24ml Calibrator 1 x 10ml
Our Abpromise guarantee covers the use of ab272521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272521 has not yet been referenced specifically in any publications.