β-Glucuronidase Activity Assay Kit (Fluorometric) (ab234625)

Overview

  • Product name

    β-Glucuronidase Activity Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Tissue, Cell Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    1 µU
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    β-Glucuronidase Activity Assay Kit (Fluorometric) (ab234625) provides a quick, reliable fluorometric method for measurements of β-Glucuronidase activities of samples and tissue lysates. The provided substrate, which is specific to β-Glucuronidases, is cleaved into a fluorescent product in the presence of β-Glucuronidase. This kit, when used according to the protocol, is sensitive enough to detect as little as one µU (1 pmol/min) of activity.

  • Notes

    β-Glucuronidases are hydrolytic enzymes responsible for the breakdown of carbohydrates.  Specifically, β-Glucuronidases cleave the terminal β-D-glucuronic acid residue from the non-reducing terminus of a mucopolysaccharide chain.  In humans, these enzymes are found in the lysosome of many tissue types.  Loss of β-Glucuronidase activity results in a metabolic disease known as Sly syndrome.  One pharmaceutical application for these enzymes is the metabolism of glucuronidated prodrugs into active pharmacological compounds.  As expression and activities of β-Glucuronidases vary substantially between tissue types and disease states, these enzymes have been used to achieve targeted activation of oncotherapeutic compounds, some of which may be toxic to healthy cells not associated with malignancy or disease.  It is thus important to have knowledge of the β-Glucuronidase activity in the tested sample to determine whether the prodrug or active form will predominate.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    β-Glucuronidase Positive Control 1 vial
    β-Glucuronidase Substrate 1 x 100µl
    β-Glucuronidase Assay Buffer 1 x 25ml
    4-Methylumbelliferone Standard (5 mM) 1 x 35µl

Images

  • Assays were performed following the kit protocol.

Protocols

References

ab234625 has not yet been referenced specifically in any publications.

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