Overview

  • Product name

    Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end
  • Description

    Rabbit polyclonal to beta Glucuronidase (GUS) - Aminoterminal end
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MLRPVETPTREIKKLD

  • Positive control

    • Cell extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing E. coli GUS.

Properties

Applications

Our Abpromise guarantee covers the use of ab50148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa. Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.
IHC-Fr Use at an assay dependent concentration. PubMed: 21825109

Target

  • Relevance

    Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Alternative names

      • Beta D glucuronidase antibody
      • Beta glucuronidase antibody
      • gurA antibody
      • UidA antibody

    References

    This product has been referenced in:

    • Cai Q  et al. Jasmonic acid regulates spikelet development in rice. Nat Commun 5:3476 (2014). WB . Read more (PubMed: 24647160) »
    • Jasik J  et al. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiol 157:563-73 (2011). IHC-Fr . Read more (PubMed: 21825109) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you very much for your question. Indeed the ab50148 is a polylconal antibody of rabbit origin. The antibody has been purified by affinity to the immunogen and the isotype has not been determined. However the rabbit immune system is only capable of producing IgG1 as an IgG and therefore this antibody will mainly consist out of IgG1. If the customer is searching for an isotype control, I can recommend to use also purified rabbit IgGs. I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

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