Overview

  • Product name
    Anti-beta glucuronidase (GUSB) antibody [105]
    See all beta glucuronidase (GUSB) primary antibodies
  • Description
    Mouse monoclonal [105] to beta glucuronidase (GUSB)
  • Host species
    Mouse
  • Specificity
    This antibody reacts with an non-active center epitope on human placental and liver Beta Glucuronidase. The antibody apparently reacts with the native enzyme only.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, WB, Othermore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat

Properties

Applications

Our Abpromise guarantee covers the use of ab8478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP
ELISA
IHC-Fr
WB
Other
  • Application notes
    AP: 1/10.
    ELISA: 1/10.
    IHC-Fr: 1/10.
    WB: 1/10. The beta-glucuronidase is in its native form a tetramer of MW 280 kDa. Furthermore it can be glycosylated.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays an important role in the degradation of dermatan and keratan sulfates.
    • Involvement in disease
      Defects in GUSB are the cause of mucopolysaccharidosis type 7 (MPS7) [MIM:253220]; also known as Sly syndrome. MPS7 is an autosomal recessive lysosomal storage disease characterized by inability to degrade glucuronic acid-containing glycosaminoglycans. The phenotype is highly variable, ranging from severe lethal hydrops fetalis to mild forms with survival into adulthood. Most patients with the intermediate phenotype show hepatomegaly, skeletal anomalies, coarse facies, and variable degrees of mental impairment.
      Note=Mucopolysaccharidosis type 7 is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 2 family.
    • Post-translational
      modifications
      N-linked glycosylated with 3 to 4 oligosaccharide chains.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ac2-223 antibody
      • asd antibody
      • Beta G1 antibody
      • Beta glucuronidase antibody
      • Beta-G1 antibody
      • Beta-glucuronidase antibody
      • BG antibody
      • BGLR antibody
      • BGLR_HUMAN antibody
      • FLJ39445 antibody
      • Glucuronidase beta antibody
      • Gur antibody
      • Gus antibody
      • Gus-r antibody
      • Gus-s antibody
      • Gus-t antibody
      • Gus-u antibody
      • GUSB antibody
      • Gut antibody
      • MPS7 antibody
      see all

    References

    ab8478 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-9 of 9 Abreviews or Q&A

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab8478. The antibody reacts with human and does not react with rat. We currently do not have any other beta Glucuronidase antibodies. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

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    Answer

    Thank you for your email. I'm replying on behalf of Sarah, who is currently out of the office. I have arranged for ab9484 to be sent to you as a free of charge replacement for ab8478. It is being sent to your attention on order# 102313 and you should receive it tomorrow. Please let me know if you have any additional questions or concerns.

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    Answer

    We have just received the following information from the originator of ab8478: The immunogen used to generate the antibody was of human origin. The antibody was not tested against bacterial beta glucuronidase. You can find more information in the following application: Haisma et al, Hybridoma, volume 14, number 4, 1995. I hope this information helps, please do not hesitate to contact us again if you need further advice,

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    Question
    Answer

    The antibody binds to circulating B-glucuronidase.

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    Answer

    Thank you for your patience. I have only just heard back from the antibody's originator. The protein in its native format is a tetramer of approximately 280 kDa. The monomer format is approximately 74 kD. This can deviate a little bit on the gel depending on the glycosilation.

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    Answer

    The MW of this antibody is 150 kD. This antibody reacts in WB with a single protein of approximately 280 kDa.

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    Answer

    Cross reactivity with E.coli beta glucuronidase has not been tested, but as the antibody does not cross react with the rat enzyme I am doubtful that it will work with a bacterial enzyme.

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    Question
    Answer

    The antibody will not cross-react with rat, so we do not think that it is likely to cross react with mouse.

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    Answer

    It ahs been tested at 1 in 10 but as you say, this is high and probably wasteful. We would therfore recommend that you titrate the antibody at 1 in 10, 1 in 50, 1 in 100 and 1 in 500 in your particular application.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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