Overview

  • Product name

    Anti-beta I Tubulin antibody [2A1A9]
    See all beta I Tubulin primary antibodies
  • Description

    Mouse monoclonal [2A1A9] to beta I Tubulin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human beta I Tubulin aa 33-166. expressed in E. Coli.
    Sequence:

    AGSDRGAS ALQLERISVY YNEAYGRKYV PRAVLVDLEP GTMDSIRSSK LGALFQPDSF VHGNSGAGNN WAKGHYTEGA ELIENVLEVV RHESESCDCL QGFQIVHSLG GGTGSGMGTL LMNKIREEYP DRIMNS


    Database link: Q9H4B7

  • Positive control

    • Transfected HEK293 cell lysate; K562, HepG2, A431, Jurakat, Hela, NIH-3T3, Cos7, PC12 cell lysates; A431 cells, ovarian cancer tissues; Hela cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2A1A9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Involvement in disease

    Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810484G07Rik antibody
    • beta 1 tubulin antibody
    • Beta tubulin 1, class VI antibody
    • Beta-tubulin antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Images

  • Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution + human beta 1 Tubulin (AA: 33-166) recombinant protein

    Predicted band size: 50 kDa

  • All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : human beta 1 Tubulin (AA: 33-166) -hIgGFc transfected HEK293 cell lysate

    Predicted band size: 50 kDa

  • All lanes : Anti-beta I Tubulin antibody [2A1A9] (ab204947) at 1/500 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Hela cell lysate
    Lane 6 : NIH-3T3 cell lysate
    Lane 7 : Cos7 cell lysate
    Lane 8 : PC12 cell lysate

    Predicted band size: 50 kDa

  • Immunofluorescent staining of Hela cells labeling beta I Tubulin with ab204947 at 1/200 (green).  Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow cytometric analysis of A431 cells labeling beta I Tubulin with ab204947 at 1/200 (green) or negative control (red)

  • Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.

  • Immunohistochemical analysis of formalin fixed paraffin embedded ovarian cancer tissue labeling beta I Tubulin using ab204947 at 1/200 dilution followed by DAB staining.

References

ab204947 has not yet been referenced specifically in any publications.

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