Overview

  • Product name

    Anti-beta II Tubulin antibody
    See all beta II Tubulin primary antibodies
  • Description

    Rabbit polyclonal to beta II Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    A recombinant protein fragment corresponding to a region within amino acids 243 and 445 of Human beta II Tubulin (UniProt ID: P68371).

  • Positive control

    • A431; NIH 3T3; Jurkat; 293T and Raji cell lysates; A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab151318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA506K6.1 antibody
    • beta 2 tubulin antibody
    • Beta2 antibody
    • class II beta tubulin isotype antibody
    • class IIa beta-tubulin antibody
    • class IIb beta-tubulin antibody
    • Class IVb beta tubulin antibody
    • dJ40E16.7 antibody
    • DKFZp566F223 antibody
    • FLJ98847 antibody
    • M(beta)2 antibody
    • MGC8685 antibody
    • OTTHUMP00000015956 antibody
    • OTTHUMP00000015964 antibody
    • TBB4B_HUMAN antibody
    • TUBB 2 antibody
    • TUBB 2A antibody
    • TUBB 2C antibody
    • TUBB antibody
    • TUBB PARALOG antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB2B antibody
    • TUBB2C antibody
    • Tubb4b antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2 chain antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A chain antibody
    • Tubulin beta 2B antibody
    • Tubulin beta 2B chain antibody
    • Tubulin beta 2C antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide paralog antibody
    • Tubulin beta-2 chain antibody
    • Tubulin beta-2C chain antibody
    • Tubulin beta-4B chain antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta 2B class IIb antibody
    • tubulin, beta 4B class IVb antibody
    • Tubulin, beta, class IVB antibody
    • Tubulin, beta-4B antibody
    see all

Images

  • Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution + A431 cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-beta II Tubulin antibody (ab151318) at 1/5000 dilution + NIH 3T3 cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells, labeling beta II Tubulin using ab151318 at 1/200 dilution.
    The image in the right panel was costained with Hoechst 33342.

References

This product has been referenced in:

  • Wu B  et al. High expression of RAD18 in glioma induces radiotherapy resistance via down-regulating P53 expression. Biomed Pharmacother 112:108555 (2019). Read more (PubMed: 30798132) »
  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
See all 12 Publications for this product

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