The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 50 kDa.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Belongs to the tubulin family.
The highly acidic C-terminal region may bind cations such as calcium.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Western blot - Anti-beta II Tubulin antibody - Aminoterminal end (ab170363)
All lanes : Anti-beta II Tubulin antibody - Aminoterminal end (ab170363)
Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : MDA-MB231 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody - Aminoterminal end (ab170363)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling beta II Tubulin (green) using ab170363. An Alexa Fluor® 488-conjugated goat anti-rabbit lgG was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody - Aminoterminal end (ab170363)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling beta II Tubulin using ab170363. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.