Heat mediated - Buffer/Enzyme Used: Citric acid HIER
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Non-Abcam antibody was used: anti Mouse IgGs Host species: Goat Clonality: Polyclonal Conjugation: Biotin
This section was cut from an anonymous autopsy P.wax block that is over 20 yrs old, so I would expect variable positivity when compared with more recently sampled tissues (giving higher dilution factors, such as I obtained with fresh mouse/rat CNS blocks). Transverse-cut axons stain very intensely longitudinal nerve processes and cell bodies are not so heavily stained but are still easily seen.
Many thanks for your feedback on ab78078 (Neuron specific beta III Tubulin antibody [2G10] - Neuronal Marker) on human brain tissue in IHC-P.
Mr. Carl Hobbs
Submitted Apr 21 2010
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