Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuron specific beta III Tubulin antibody [2G10] - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (medulla oblongata)
Specification
medulla oblongata
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid HIER
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Mouse IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
This section was cut from an anonymous autopsy P.wax block that is over 20 yrs old, so I would expect variable positivity when compared with more recently sampled tissues (giving higher dilution factors, such as I obtained with fresh mouse/rat CNS blocks). Transverse-cut axons stain very intensely longitudinal nerve processes and cell bodies are not so heavily stained but are still easily seen.
Abcam response
Many thanks for your feedback on ab78078 (Neuron specific beta III Tubulin antibody [2G10] - Neuronal Marker) on human brain tissue in IHC-P.

Mr. Carl Hobbs

Verified customer

Submitted Apr 21 2010

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