Thia antibody produced (diluted 1:300) an intense and widespread staining in axons in many brain areas such as cortex, hippocampus. Excellent +++ the pictures loaded show the staining onobtained in the rat hippocampus, at low (right panel, objective X20) and high power magnification (left panel).
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Dr. Sophie Pezet
Submitted Sep 08 2010
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