Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuron specific beta III Tubulin antibody [2G10] - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain - Hippocampus)
Specification
Brain - Hippocampus
Fixative
Paraformaldehyde

Other product details

Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Thia antibody produced (diluted 1:300) an intense and widespread staining in axons in many brain areas such as cortex, hippocampus. Excellent +++
the pictures loaded show the staining onobtained in the rat hippocampus, at low (right panel, objective X20) and high power magnification (left panel).

The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010

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