Western blot abreview for Anti-beta III Tubulin antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
50 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: anti-Rabbit IgG-HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL prime
Exposure
10 second(s)
Bands
Specific: 55 kDa Non-specific: 40 kDa
Positive control
liver and brain

Abcam user community

Verified customer

Submitted Nov 13 2017

Sign up