Western blot abreview for Anti-beta III Tubulin antibody

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Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-tubulin
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50, 48 kDa
Positive control
Rat brain

Additional data

Additional Notes
beta-III-tubulin: 50kDa tyrosine hydroxylase: 48kDa Lane 1 + 3: Rat striatum lysate, samples. Lane 2: Rat brain lysate, control.

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Submitted Jun 27 2018

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