Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Vectorlabs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Not quite as sharp results as with TUJ1 but, as far as I can determine, the pattern of positivity is the same as TUJ1. The picture I have submittted highlights the primary processes of Purkinje cells: at a lower dilution factor the other neuronal processes in the molecular layer are demonstrated very well. Photoshop - enhanced

Mr. Carl Hobbs

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Submitted Mar 05 2007

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