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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)

Reviews (1) Submit a question References (13)
Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
  • Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1568Y] to beta III Tubulin
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE

Overview

  • Product name

    Anti-beta III Tubulin antibody [EPR1568Y]
    See all beta III Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR1568Y] to beta III Tubulin

  • Host species

    Rabbit

  • Specificity

    according to BLAST analysis, it is possible that the antibody will cross-react with TBB6 (Q9BUF5), TBB2B (Q9BVA1), TBB2A (Q13885), TBB5 (P07437), TBB4A (P04350), TBB4B (P68371). This cross-reactivity has not been confirmed experimentally.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human beta III Tubulin aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y, HeLa, Human cerebellum, Mouse brain and Rat brain lysate Flow Cyt: HeLa cells ICC/IF: Neuro-2a cells, Hap1-TUBB3 WT/KO IHC-P: Human tonsil, Mouse and rat testis tissue sections

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1568Y

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.

For the unpurified format use  at 1-5 µg/ml
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For the unpurified format use at 1/1000 dilution
WB
1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

For the unpurified format use at 1/20000 to 1/100,000 dilution
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Fot the unpurified format use at 1/250 to 1/500  dilution
Notes
ICC/IF
1/50.

For the unpurified format use  at 1-5 µg/ml
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For the unpurified format use at 1/1000 dilution
WB
1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

For the unpurified format use at 1/20000 to 1/100,000 dilution
IHC-P
1/8000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Fot the unpurified format use at 1/250 to 1/500  dilution

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

  • Tissue specificity

    Expression is primarily restricted to central and peripheral nervous system.

  • Involvement in disease

    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • beta 3 tubulin antibody
    • beta 4 antibody
    • beta-4 antibody
    • CDCBM antibody
    • CDCBM1 antibody
    • CFEOM3 antibody
    • CFEOM3A antibody
    • FEOM3 antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron specific beta III Tubulin antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • Tubb 3 antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    • tuj 1 antibody
    • tuj1 antibody
    see all

Images

  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Anti-beta III Tubulin antibody [EPR1568Y] (ab68193) at 1/50000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    All lanes : Anti-beta III Tubulin antibody [EPR1568Y] (ab68193) at 1/50000 dilution (Purified)

    Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : Human cerebellum lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Flow Cytometry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling beta III Tubulin with Purified ab68193 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunocytochemistry - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunocytochemistry analysis of Neuro-2a(Mouse neuroblastoma neuroblast) cells labeling beta III Tubulin with Purified ab68193 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling beta III Tubulin with Purified ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling beta III Tubulin with Purified ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling beta III Tubulin with Purified ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)

    ab68193 staining beta III Tubulin in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab68193 at 1μg/ml concentration and ab7291, a mouse anti-tubulin antibody, at 1μg/ml overnight at +4°C. This is followed by a further incubation at room temperature for 1h with a goat anti-rabbit IgG Alexa Fluor® 488 (ab150081) at 2μg/ml (shown in green) and a goat anti-mouse IgG Alexa Fluor® 647 (ab150119) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)
    Anti-beta III Tubulin antibody [EPR1568Y] (ab68193)

References (13)

Publishing research using ab68193? Please let us know so that we can cite the reference in this datasheet.

ab68193 has been referenced in 13 publications.

  • Shan W  et al. Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells. Exp Ther Med 21:619 (2021). PubMed: 33936276
  • Tiwari R  et al. Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer. Nat Commun 11:384 (2020). PubMed: 31959826
  • Zhang C  et al. Protopanaxadiol ginsenoside Rd protects against NMDA receptor-mediated excitotoxicity by attenuating calcineurin-regulated DAPK1 activity. Sci Rep 10:8078 (2020). PubMed: 32415270
  • Morales-Garcia JA  et al. N,N-dimethyltryptamine compound found in the hallucinogenic tea ayahuasca, regulates adult neurogenesis in vitro and in vivo. Transl Psychiatry 10:331 (2020). PubMed: 32989216
  • Boldyreva MA  et al. Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle. Int J Mol Sci 20:N/A (2019). PubMed: 31238604
View all Publications for this product

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IHC - Wholemount abreview for Anti-beta III Tubulin antibody [EPR1568Y]

 Good
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Asytanax mexicanus Tissue (gut of 8.5 days post fertilization fish)
Specification
gut of 8.5 days post fertilization fish
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted May 25 2022

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