Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cerebellum)
Antigen retrieval step
Heat mediated
Specification
cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Vectorlabs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Not quite as sharp results as with TUJ1 but, as far as I can determine, the pattern of positivity is the same as TUJ1. The picture I have submittted highlights the primary processes of Purkinje cells: at a lower dilution factor the other neuronal processes in the molecular layer are demonstrated very well. Photoshop - enhanced
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
Submitted Mar 05 2007