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I am using two of your products (nestin and beta III) for phenotype evaluation
in neural stem cell culture, based on recommended dilutions and with the
secondary antibodies listed in the attached slides. However as shown in the
second slide (please see the attachment) both products stain whole cells (even
nucleus) instead of staining the appropriate part. Please advise me what to do
to not have this problem.
Asked on Mar 14 2012
Thank you for contacting Abcam.
I am sorry that you have been experiencing difficulties with these antibodies in ICC. I have reviewed the images provided, but I was wondering if you would also provide a detailed summary of your protocol and sample preparation. This will help me to evaluate if there are any suggestions I can make to improve your results or if the antibodies are faulty.
I look forward to your reply so that I may assist you further. Please do not hesitate to contact me with any additional questions or concerns.
Answered on Mar 14 2012