Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuron specific beta III Tubulin antibody [TU-20]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Composite HP image contrasts clone TU20 and 2G10 positivity in Ganglia of Auerbach's plexus ( between the inner circular and outer longitudinal smooth muscle layers of colon: the section is obliquely cut, thus clear demarcation between layers is lost).
In my humble opinion, so far, clone 2G10 is superior to clone TU20.
Of course, I could double the concentration of TU20 antibody to attempt to achieve the same signal:noise ratio but, I would only do this if I have only purchased TU20.

Mr. Carl Hobbs

Verified customer

Submitted Nov 12 2009

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