Immunohistochemistry (Frozen sections) abreview for Anti-Neuron specific beta III Tubulin antibody [TU-20]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Whole embyro: e12)
Specification
Whole embyro: e12
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
Floorplate region of developing cervical spinal cord, I think (I have not studied this particular region). ab78078, ab14545 and ab18207 appear to give very similar results on sequential sections
Abcam response
Many thanks for this feedback on ab7751 (Neuron specific beta III Tubulin antibody [TU-20]) in IHC-P on mouse embryo (spinal) sections.

Mr. Carl Hobbs

Verified customer

Submitted Apr 21 2010

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