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    beta-iii-tubulin-antibody-tu-20-neuronal-marker-ab7751.pdf

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Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

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Reviews (23)Q&A (15)References (144)

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Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Western blot - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
  • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

Key features and details

  • Mouse monoclonal [TU-20] to beta III Tubulin - Neuronal Marker
  • Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker
    See all beta III Tubulin primary antibodies
  • Description

    Mouse monoclonal [TU-20] to beta III Tubulin - Neuronal Marker
  • Host species

    Mouse
  • Specificity

    Class III beta-tubulin specific for neurons.

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species, Mammals
  • Immunogen

    Synthetic peptide corresponding to Human beta III Tubulin aa 400-500 conjugated to keyhole limpet haemocyanin.
    Database link: Q13509

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    ESESQGPK (aa 441-448)
  • Positive control

    • ICC: Neuro-2a, P-19, Primary mouse cortical culture and Induced neurons derived from human pluripotent cells. WB: Human brain and spinal cord tissue lysate. IHC-P: Human cerebellum tissue. Flow Cyt (Intra): SH-SY5Y cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    TU-20
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • FITC Anti-beta III Tubulin antibody [TU-20] (ab25770)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human beta III Tubulin protein (ab140581)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
Use a concentration of 1 - 2 µg/ml. We suggest reducing conditions and a 90 minute incubation time.
Flow Cyt (Intra)
1/20 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (7)
Use a concentration of 10 µg/ml.
ICC
1/1000.
Notes
WB
Use a concentration of 1 - 2 µg/ml. We suggest reducing conditions and a 90 minute incubation time.
Flow Cyt (Intra)
1/20 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 10 µg/ml.
ICC
1/1000.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • Tissue specificity

    Expression is primarily restricted to central and peripheral nervous system.
  • Involvement in disease

    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession Q13509 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10381 Human
    • Entrez Gene: 22152 Mouse
    • Omim: 602661 Human
    • SwissProt: Q13509 Human
    • SwissProt: Q9ERD7 Mouse
    • Unigene: 511743 Human
    • Unigene: 40068 Mouse
    • Alternative names

      • beta 3 tubulin antibody
      • beta 4 antibody
      • beta-4 antibody
      • CDCBM antibody
      • CDCBM1 antibody
      • CFEOM3 antibody
      • CFEOM3A antibody
      • FEOM3 antibody
      • M(beta)3 antibody
      • M(beta)6 antibody
      • MC1R antibody
      • Neuron specific beta III Tubulin antibody
      • Neuron-specific class III beta-tubulin antibody
      • QccE-11995 antibody
      • QccE-15186 antibody
      • TBB3_HUMAN antibody
      • Tubb 3 antibody
      • TUBB3 antibody
      • TUBB4 antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 3 chain antibody
      • Tubulin beta 4 antibody
      • Tubulin beta III antibody
      • Tubulin beta-3 chain antibody
      • Tubulin beta-4 chain antibody
      • Tubulin beta-III antibody
      • tuj 1 antibody
      • tuj1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

      Immunocytochemistry staining of mouse Neuro2a cells (neuroblast/neuronal and amoeboid stem cells) using 3µg/ml ab7751 (green), nuclear counterstaining with DAPI (blue).

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

      Immunocytochemistry staining of mouse P-19 cells (embryonal carcinoma).

      A: Microtubules stained with neuron-specific ab7751 in red. 

      B: Merged image of co-staining with ab7751 and anti-beta-tubulin.

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)Ganz et al PLoS One. 2014 Jun 19;9(6):e100445. doi: 10.1371/journal.pone.0100445. eCollection 2014. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Naive hOMSC express constitutively neuronal and dopaminergic markers.

      Immunofluorescence of neuronal (A–C) and dopaminergic (D–F) markers in naïve hOMSC, scale bars 100 µM.

      Cells obtained from two donors (24 and 35 years old) were fixed in 4% PFA-PBS and pre-incubated for 60 min in blocking solution (5% goat serum, 1% BSA, 0.05% Triton-X in PBS). Primary antibodies were diluted in the blocking solution and applied overnight at 4°C. The following primary antibodies were used, β-III-Tubulin (1∶200, ab7751, abcam), Map2 (1∶200), synapsin (1∶200), tyrosine hydroxylase (1∶200), Lmx1A (1∶200), Pitx3 (1∶200) and Nurr1 (1∶200).

      Primary antibodies were detected with fluorescent-labeled secondary antibodies Alexa®488 and 568 (1∶500,) for 1 h at room temperature. Nuclear DNA was stained using 4,6-diamino-2-phenylindole (DAPI) (1∶1000).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)Sun et al PLoS One. 2017 Jun 7;12(6):e0179047. doi: 10.1371/journal.pone.0179047. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Kif2a is involved in the differentiation of NSCs/NPCs.

      (Panel A) Mouse embryos were electroporated with indicated plasmids (scramble shRNA, Kif2a shRNA1 or Kif2a shRNA1+Kif2aRes) at E13.5, and analyzed at E15.5. GFP (green) represents cells expressing the indicated plasmids; TU20 (ab7751, red) represents immature neurons. Scale bars = 50 μm.

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)Gonzales et al PLoS One. 2018 Mar 27;13(3):e0194581. doi: 10.1371/journal.pone.0194581. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Confirmation of differentiation of iPSCs into cortical neuronal cultures.

      Images taken from clones C2 (trisomic) and C3 -D21 (disomic) 40 days after initiation of the differentiation protocol. Fixed cells on chamber slides were probed with antibodies against the marker proteins listed in the left column. Neuronal marker Beta III tubulin is red (ab7751); DAPI is blue; and Ctip2, TBR1, SLC17A7 (vGLUT1) and GRIK2 are green. Size bar = 20 μ.

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

      ab7751 staining beta III Tubulin in Neuro-2a (Mouse neuroblastoma cell line) cells.

      The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7751 at 1/1000 and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Rabbit secondary (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.
      Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

    • Western blot - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Western blot - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      All lanes : Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751) at 1 µg/ml

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human spinal cord tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : IRDye® 800CW Goat Anti-Mouse at 1/10000 dilution

      Performed under reducing conditions.

      Observed band size: 51 kDa why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using LI-COR® blocking buffer before being incubated with ab7751 overnight at 4°C. Antibody binding was detected using the IRDye® 800CW Goat Anti-Mouse secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Odyssey® CLx Imaging System.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

      IHC image of ab7751 staining beta III Tubulin in human cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7751, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)This image is courtesy of an anonymous Abreview

      ab7751 staining beta III Tubulin in induced neurons derived from human pluripotent cells by ICC (Immunocytochemistry).

      Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)

      Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab7751 (red line). 

      The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7751, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

      This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

      ab7751 staining neuron specific beta III Tubulin in human colon tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in citric acid pH 6 and then blocking with 1% BSA was performed for 10 minutes at RT. The primary antibody was used at dilution at 1/200 and incubated with sample in TBS/BSA/azide for 2 hours. A biotin conjugated goat polyclonal to mouse IgG was used at dilution at 1/200 as secondary antibody. Images A and B represent staining of the clone TU20 and 2G10 respectively in ganglia of Auerbach's plexus.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

      Immunohistochemistical detection of neuron specific beta III Tubulin using antibody (ab7751) on whole mouse e12 embryo (formaldehyde fixed/frozen sections).

      Permeabilization: No. Blocking step: 1% BSA for 10 mins @ rt°C. Primary Antibody Dilution 1/200; Incubation time 2 hours in TBS/BSA/azide. Secondary antibody: anti Mouse IgG Conjugated to Alexa Fluor® 594 (1/1000).

      Floorplate region of developing cervical spinal cord (I have not studied this particular region).

      See Abreview

    • Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)
      Immunocytochemistry - Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab7751)Photo courtesy of QBMCellScience

      ab7751 immunostaining (1/500) neuronal processes in primary mouse cortical culture.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (144)

    Publishing research using ab7751? Please let us know so that we can cite the reference in this datasheet.

    ab7751 has been referenced in 144 publications.

    • Wang Z  et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370
    • Li C  et al. SRPS associated protein WDR60 regulates the multipolar-to-bipolar transition of migrating neurons during cortical development. Cell Death Dis 12:75 (2021). PubMed: 33436552
    • van Erp S  et al. Age-related loss of axonal regeneration is reflected by the level of local translation. Exp Neurol 339:113594 (2021). PubMed: 33450233
    • Krepela E  et al. Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment. Int J Mol Sci 22:N/A (2021). PubMed: 33494271
    • Ebadi R  et al. Fndc5 knockdown significantly decreased the expression of neurotrophins and their respective receptors during neural differentiation of mouse embryonic stem cells. Hum Cell 34:847-861 (2021). PubMed: 33683654
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 38 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Dog Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TritonX100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 26 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Horse Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TritonX-100
    Specification
    Spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 25 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Neurons)
    Permeabilization
    Yes - 0.3% Triton
    Specification
    Neurons
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kate Hawkins

    Verified customer

    Submitted Jul 13 2017

    Western blot abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    5 µg
    Specification
    Hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sergi Bayod

    Verified customer

    Submitted Aug 03 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (induced Neuron derived from pluripotent cells)
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Specification
    induced Neuron derived from pluripotent cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 16 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (differentiated cultured mouse neural stem cells)
    Permeabilization
    Yes - 0.2% triton x 100 in PBS
    Specification
    differentiated cultured mouse neural stem cells
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Western blot abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (in vitro differentiated neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Loading amount
    5 µg
    Specification
    in vitro differentiated neural stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (primary cortical neuron)
    Permeabilization
    Yes - 0.1% Triton/1% BSA/PBS
    Specification
    primary cortical neuron
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 06 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Dorsal root ganglion culture)
    Permeabilization
    Yes - Methanol
    Specification
    Dorsal root ganglion culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Heiko Locher

    Verified customer

    Submitted Sep 23 2013

    1-10 of 38 Abreviews or Q&A

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