Key features and details
- Rabbit polyclonal to beta III Tubulin (phospho S172)
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-beta III Tubulin (phospho S172) antibody
See all beta III Tubulin primary antibodies
DescriptionRabbit polyclonal to beta III Tubulin (phospho S172)
SpecificityThe ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Synthetic phospho peptide corresponding to amino acid residues around serine 172 of human beta III Tubulin conjugated to KLH
- C2C12 cells; purified brain tubulin treated with ERK2 kinase.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier).
Our Abpromise guarantee covers the use of ab76286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 38 kDa.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Tissue specificityExpression is primarily restricted to central and peripheral nervous system.
Involvement in diseaseDefects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
Image 1: untreated.
Image 2: in the presence of the phospho peptide.
All lanes : Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution
Lane 1 : purified brain tubulin, untreated
Lane 2 : purified brain tubulin, treated with ERK2 kinase to phosphorylate
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab76286 has been referenced in 3 publications.
- Chang JH et al. Generation of Functional Dopaminergic Neurons from Reprogramming Fibroblasts by Nonviral-based Mesoporous Silica Nanoparticles. Sci Rep 8:11 (2018). PubMed: 29311646
- Ori-McKenney KM et al. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron 90:551-63 (2016). PubMed: 27112495
- Li WF et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). PubMed: 24728101