Anti-beta IV Tubulin antibody (ab222822)
Key features and details
- Rabbit polyclonal to beta IV Tubulin
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta IV Tubulin antibody
See all beta IV Tubulin primary antibodies -
Description
Rabbit polyclonal to beta IV Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey -
Immunogen
Recombinant full length protein corresponding to Human beta IV Tubulin aa 1 to the C-terminus.
Database link: P04350 -
Positive control
- WB: 293T and HeLa whole cell lysates. IP: HeLa cell whole lysate. ICC/IF: MCF7 cells. IHC-P: Human ovarian cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IP |
1/200 - 1/2000.
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WB |
1/500 - 1/5000. Detects a band of approximately 50, 58 kDa (predicted molecular weight: 50 kDa).
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IHC-P | (1) |
1/20 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
IP
1/200 - 1/2000. |
WB
1/500 - 1/5000. Detects a band of approximately 50, 58 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 540236 Cow
- Entrez Gene: 101865362 Cynomolgus monkey
- Entrez Gene: 10382 Human
- Entrez Gene: 22153 Mouse
- Omim: 602662 Human
- SwissProt: Q3ZBU7 Cow
- SwissProt: Q4R4X8 Cynomolgus monkey
- SwissProt: P04350 Human
see all -
Alternative names
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
see all
Images
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All lanes : Anti-beta IV Tubulin antibody (ab222822) at 1/500 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 50 kDa
Observed band size: 50,58 kDa why is the actual band size different from the predicted? -
MCF7 (human breast adenocarcinoma cell line) cells stained for beta IV Tubulin (green) using ab222822 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human ovarian cancer tissue stained for beta IV Tubulin using ab222822 at 1/100 dilution in immunohistochemical analysis.
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beta IV Tubulin was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222822 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab222822 at 1/500 dilution.
Lane 1: Rabbit monoclonal IgG (1 µg) instead of ab222822 in Hela whole cell lysate.Lane 2: ab222822 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 μg).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222822 has not yet been referenced specifically in any publications.